CAJ | 학술논문

本试验以阿维菌素染毒原代培养的王鸽脑神经细胞为模型,观察阿维菌素对神经细胞凋亡的毒性作用和一氧化氮产生量的影响.采用原位末端标记法观察细胞凋亡,四唑盐比色法检测神经细胞相对存活率,流式细胞术检测凋亡细胞百分比,分光光度法测定细胞一氧化氮的产生量.结果显示,阿维菌素可诱导神经细胞凋亡和细胞坏死,并具有随着阿维菌素剂量递增而增加的趋势,同时阿维菌素也能引起一氧化氮产生量降低,但是未发现存在剂量效应关系.
본시험이아유균소염독원대배양적왕합뇌신경세포위모형,관찰아유균소대신경세포조망적독성작용화일양화담산생량적영향.채용원위말단표기법관찰세포조망,사서염비색법검측신경세포상대존활솔,류식세포술검측조망세포백분비,분광광도법측정세포일양화담적산생량.결과현시,아유균소가유도신경세포조망화세포배사,병구유수착아유균소제량체증이증가적추세,동시아유균소야능인기일양화담산생량강저,단시미발현존재제량효응관계.
The effects of apoptosis and NO level were explored with model of AVM on king pigeon brain neurocytes in vitro culture.Apoptosis was observed by TUNEL,the relative survival rate of neurocytes was detected by MTT,the percentage of apoptotic cells was detected by Flow cytometry,and the production of NO in cells was acquired by absorption spectrometry.The results showed that AVM can induce apoptosis and cytonecrosis of neurocyte,and has increased with the dose of AVM.AVM can reduced the production of NO,too,but no dose-response relationship.