CAJ | 학술논문

目的研究神经下细胞(NSCs)移植对大鼠脊髓损伤(SCI)后红核神经元的作用.方法 5-溴脱氧尿嘧啶核苷(BrdU)法标记处于对数生长期的NSCs,采用电控脊髓损伤打击装置制作大鼠脊髓损伤模型.实验分为3组:NSCs组、SCI组和假手术组(Sham组).SCI后3 d进行NSCs移植,用免疫组化法观察移植细胞的存活及迁移情况,用辣根过氧化物酶(HRP)逆行示踪技术标记红核神经元,并用四甲基联苯胺(TMB)呈色反应显示红核脊髓束神经元的存活情况,用行为学(BBB)评分法观察大鼠瘫痪肢体的恢复情况.结果在损伤脊髓区域可检测到BrdU标记的阳性NSCs,中脑HRP标记红核神经元数目明显多于SCI组(P<0.01),BBB评分亦明显高于SCI组(P<0.01).结论体外培养的胚胎大鼠NSCs在移植到脊髓损伤区域后可存活和迁移,对SCI后巾脑红核神经元具有保护作用,从而促进了大鼠肢体功能的恢复.
목적 연구신경하세포(NSCs)이식대대서척수손상(SCI)후홍핵신경원적작용.방법 5-추탈양뇨밀정핵감(BrdU)법표기처우대수생장기적NSCs,채용전공척수손상타격장치제작대서척수손상모형.실험분위3조:NSCs조、SCI조화가수술조(Sham조).SCI후3 d진행NSCs이식,용면역조화법관찰이식세포적존활급천이정황,용랄근과양화물매(HRP)역행시종기술표기홍핵신경원,병용사갑기련분알(TMB)정색반응현시홍핵척수속신경원적존활정황,용행위학(BBB)평분법관찰대서탄탄지체적회복정황.결과 재손상척수구역가검측도BrdU표기적양성NSCs,중뇌HRP표기홍핵신경원수목명현다우SCI조(P<0.01),BBB평분역명현고우SCI조(P<0.01).결론 체외배양적배태대서NSCs재이식도척수손상구역후가존활화천이,대SCI후건뇌홍핵신경원구유보호작용,종이촉진료대서지체공능적회복.
Objective To study the effects of embryonic neural stem cells transplantion on trauma of red nucleus neu-rons of the rats with spinal cord injury.Methods NSCs in logarithmic phage were labeled with BrdU,a Sprague Dawley rat mode of spinal cord injury (SCI) was developed with electrocircuit control spinal cord injuring device.Thirty SD rats were randomly divided into three groups: sham group,SCI group and NSC group.The NSCs were trans-planted into injured site three days after SCI.Then NSCs labeled with Brdu were detected by immunohistochemisty,rubrospinal tract (RST) neurons were labeled by retrograde transport of the horseradish peroxidase (HRP) from the lesion site,which were taken by damaged axons and remained in the neurons,then the labeled red nucleus (RN) neurons were counted.Hind limb function of experimental rats was evaluated by a blinder observer using BBB open field locomotion rating score.Results BrdU positive NSCs were detected in the spinal cord after transplantation,the number of RST neurons labeled by HRP in NSC group was more than that in SCI group (P <0.01),the BBB score of NSC group was higher than SCI group (P <0.01).Conclusion The transplanted NSCs can survive in the injured site of spinal cord and protect RN,then promote more remarkably functional recovery after SCI.