农业科学与技术(英文版)
農業科學與技術(英文版)
농업과학여기술(영문판)
AGRICULTURAL SCIENCE & TECHNOLOGY
2010年
4期
64-68
,共5页
李叶峰%盛亚丹%王彩晨%王宁%陆小平
李葉峰%盛亞丹%王綵晨%王寧%陸小平
리협봉%성아단%왕채신%왕저%륙소평
勋章菊%愈伤组织%植株再生%苯基噻二唑基脲
勛章菊%愈傷組織%植株再生%苯基噻二唑基脲
훈장국%유상조직%식주재생%분기새이서기뇨
Cazania rigens L.%Callus%Plantlet regeneration%TDZ
[目的]研究勋章菊叶片再生技术,筛选适合勋章菊叶片再生的最佳培养配方.[方法]以日本引进的勋章菊叶片为材料,置床于添加不同种类和浓度激素的MS培养基中进行愈伤组织和不定芽诱导的正交试验,筛选勋章菊叶片再生的最佳培养基配方.[结果]在MS+ TDZ(0.8~1.0 mg/L)+NAA(0.05~1.0 mg/L)培养基上可形成紧密型绿色的愈伤组织;叶片接种于MS+ TDZ(0.5~1.0 mg/L)+NAA(0.05~1.0 mg/L)培养基上可形成许多不定芽,诱导效率达100%;健壮的不定芽长至2.0~3.0 cm长时移至1/2 MS+NAA(0.1 mg/L)培养基中,其发根状况良好,生根率达100%.[结论]为勋章菊的高频快繁提供了新途径,并为勋章菊的遗传转化及培育新品种奠定基础.
[目的]研究勛章菊葉片再生技術,篩選適閤勛章菊葉片再生的最佳培養配方.[方法]以日本引進的勛章菊葉片為材料,置床于添加不同種類和濃度激素的MS培養基中進行愈傷組織和不定芽誘導的正交試驗,篩選勛章菊葉片再生的最佳培養基配方.[結果]在MS+ TDZ(0.8~1.0 mg/L)+NAA(0.05~1.0 mg/L)培養基上可形成緊密型綠色的愈傷組織;葉片接種于MS+ TDZ(0.5~1.0 mg/L)+NAA(0.05~1.0 mg/L)培養基上可形成許多不定芽,誘導效率達100%;健壯的不定芽長至2.0~3.0 cm長時移至1/2 MS+NAA(0.1 mg/L)培養基中,其髮根狀況良好,生根率達100%.[結論]為勛章菊的高頻快繁提供瞭新途徑,併為勛章菊的遺傳轉化及培育新品種奠定基礎.
[목적]연구훈장국협편재생기술,사선괄합훈장국협편재생적최가배양배방.[방법]이일본인진적훈장국협편위재료,치상우첨가불동충류화농도격소적MS배양기중진행유상조직화불정아유도적정교시험,사선훈장국협편재생적최가배양기배방.[결과]재MS+ TDZ(0.8~1.0 mg/L)+NAA(0.05~1.0 mg/L)배양기상가형성긴밀형록색적유상조직;협편접충우MS+ TDZ(0.5~1.0 mg/L)+NAA(0.05~1.0 mg/L)배양기상가형성허다불정아,유도효솔체100%;건장적불정아장지2.0~3.0 cm장시이지1/2 MS+NAA(0.1 mg/L)배양기중,기발근상황량호,생근솔체100%.[결론]위훈장국적고빈쾌번제공료신도경,병위훈장국적유전전화급배육신품충전정기출.
[Objective] The research aimed to study the regeneration technology of Cazania rigens L. leaves and screen out the optimum medium formula for the regeneration of Cazania rigens L. leaves. [Method] Using Japan imported C. rigens leaves as materials, the orthogonal test was made for the callus and adventitious buds induction in MS medium with different kinds and concentrations of hormones. The optimum medium formula for the regeneration of C. rigens leaves were screened out. [Result] On the medium of MS+ 0.8-1.0 mg/L TDZ + 0.05-1.0 mg/L NAA, compact type and bright green calli were formed. When the leaves were inoculated on the medium of MS+ 0.5-1.0 mg/L TDZ + 0.05-1.0 mg/L NAA, many adventitious shoots can be induced and the induction rate reached 100%. When strong adventitious shoots with the height of 2.0-3.0 cm were transplanted into the medium of 1/2 MS +0.1 mg/L NAA, the rooting situations were good and the rooting rate was 100%. [Conclusion] The research provided a new way for the rapid propagation of C. rigens and laid the foundation for the genetic transformation and new varieties breeding of C. rigens.