中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2008年
2期
166-169
,共4页
滑膜肉瘤%p27%增殖细胞核抗原%增殖相关核抗原%基因表达
滑膜肉瘤%p27%增殖細胞覈抗原%增殖相關覈抗原%基因錶達
활막육류%p27%증식세포핵항원%증식상관핵항원%기인표체
Synovial sarcoma%p27%Proliferating cell nuclear antigen%Nuclear-associated antigen%Gene expressions
目的 观察p27、增殖细胞核抗原(PCNA)、增殖相关核抗原(ki-67)在滑膜肉瘤中的表达,探讨三者的相互关系及对预后的影响.方法 10例正常滑膜和36例滑膜肉瘤石蜡切片标本,采用免疫组化(SP)方法,观察、p27,PCNA、ki-67在正常滑膜和滑膜肉瘤中的表达;通过对比分析,比较p27、PCNA、ki-67在滑膜肉瘤表达中的相关性;根据滑膜肉瘤镜下组织学分级、组织学分型和临床分期,分析p27、PCNA、ki-67表达与预后的关系.结果 ①正常滑膜组织中p27呈高表达[100%(10/10)],PCNA和ki-67无表达(0、0);滑膜肉瘤中p27[11.1%(4/36)]呈低表达,PCNA[86.1%(31/36)]和ki-67[69.4%(25/36)]呈高表达;p27、PCNA、ki-67在正常滑膜和滑膜肉瘤之间的表达比较差异有统计学意义(χ2值分别为25.16、22.63、12.54,P＜0.05).②p27表达与滑膜肉瘤的组织学分级、预后有关(χ2值分别为9.19、5.98,P＜0.05),与组织学类型、临床分期无关(χ2值分别为0.77、0.29,P＞0.05);PCNA和ki-67的表达与滑膜肉瘤的临床分期、组织学分级、预后有关(χ2值分别为7.40、8.12,7.17、9.18,13.03、10.66,P＜0.05),与组织学类型无关(χ2值分别为0.64、0.36,P＞0.05).③p27与PCNA表达呈负相关(r=-0.63,P＜0.05),p27与ki-67表达呈负相关(r=-0.53,P＜0.05),PCNA与ki-67的表达呈正相关(r=0.63,P＞0.05).结论 p27在滑膜肉瘤组织中表达缺失,PCNA和ki-67在滑膜肉瘤中呈高表达,滑膜肉瘤细胞增殖过程中p27与PCNA、ki-67互相调节共同发挥作用,联合检测p27和PCNA、ki-67表达对判断滑膜肉瘤患者的预后更有价值.
目的 觀察p27、增殖細胞覈抗原(PCNA)、增殖相關覈抗原(ki-67)在滑膜肉瘤中的錶達,探討三者的相互關繫及對預後的影響.方法 10例正常滑膜和36例滑膜肉瘤石蠟切片標本,採用免疫組化(SP)方法,觀察、p27,PCNA、ki-67在正常滑膜和滑膜肉瘤中的錶達;通過對比分析,比較p27、PCNA、ki-67在滑膜肉瘤錶達中的相關性;根據滑膜肉瘤鏡下組織學分級、組織學分型和臨床分期,分析p27、PCNA、ki-67錶達與預後的關繫.結果 ①正常滑膜組織中p27呈高錶達[100%(10/10)],PCNA和ki-67無錶達(0、0);滑膜肉瘤中p27[11.1%(4/36)]呈低錶達,PCNA[86.1%(31/36)]和ki-67[69.4%(25/36)]呈高錶達;p27、PCNA、ki-67在正常滑膜和滑膜肉瘤之間的錶達比較差異有統計學意義(χ2值分彆為25.16、22.63、12.54,P＜0.05).②p27錶達與滑膜肉瘤的組織學分級、預後有關(χ2值分彆為9.19、5.98,P＜0.05),與組織學類型、臨床分期無關(χ2值分彆為0.77、0.29,P＞0.05);PCNA和ki-67的錶達與滑膜肉瘤的臨床分期、組織學分級、預後有關(χ2值分彆為7.40、8.12,7.17、9.18,13.03、10.66,P＜0.05),與組織學類型無關(χ2值分彆為0.64、0.36,P＞0.05).③p27與PCNA錶達呈負相關(r=-0.63,P＜0.05),p27與ki-67錶達呈負相關(r=-0.53,P＜0.05),PCNA與ki-67的錶達呈正相關(r=0.63,P＞0.05).結論 p27在滑膜肉瘤組織中錶達缺失,PCNA和ki-67在滑膜肉瘤中呈高錶達,滑膜肉瘤細胞增殖過程中p27與PCNA、ki-67互相調節共同髮揮作用,聯閤檢測p27和PCNA、ki-67錶達對判斷滑膜肉瘤患者的預後更有價值.
목적 관찰p27、증식세포핵항원(PCNA)、증식상관핵항원(ki-67)재활막육류중적표체,탐토삼자적상호관계급대예후적영향.방법 10례정상활막화36례활막육류석사절편표본,채용면역조화(SP)방법,관찰、p27,PCNA、ki-67재정상활막화활막육류중적표체;통과대비분석,비교p27、PCNA、ki-67재활막육류표체중적상관성;근거활막육류경하조직학분급、조직학분형화림상분기,분석p27、PCNA、ki-67표체여예후적관계.결과 ①정상활막조직중p27정고표체[100%(10/10)],PCNA화ki-67무표체(0、0);활막육류중p27[11.1%(4/36)]정저표체,PCNA[86.1%(31/36)]화ki-67[69.4%(25/36)]정고표체;p27、PCNA、ki-67재정상활막화활막육류지간적표체비교차이유통계학의의(χ2치분별위25.16、22.63、12.54,P＜0.05).②p27표체여활막육류적조직학분급、예후유관(χ2치분별위9.19、5.98,P＜0.05),여조직학류형、림상분기무관(χ2치분별위0.77、0.29,P＞0.05);PCNA화ki-67적표체여활막육류적림상분기、조직학분급、예후유관(χ2치분별위7.40、8.12,7.17、9.18,13.03、10.66,P＜0.05),여조직학류형무관(χ2치분별위0.64、0.36,P＞0.05).③p27여PCNA표체정부상관(r=-0.63,P＜0.05),p27여ki-67표체정부상관(r=-0.53,P＜0.05),PCNA여ki-67적표체정정상관(r=0.63,P＞0.05).결론 p27재활막육류조직중표체결실,PCNA화ki-67재활막육류중정고표체,활막육류세포증식과정중p27여PCNA、ki-67호상조절공동발휘작용,연합검측p27화PCNA、ki-67표체대판단활막육류환자적예후경유개치.
Objective To investigate the association of the expressions of p27 and proliferating cell nuclear antigen(PCNA)and nuclear-associated antigen(ki-67)proteins in synovial sarcoma,and the relationship with its prognosis.Methods The expression of p27,PCNA and ki-67 in 36 synovial sarcoma and 10 normal synovial tissue were immunohistochemically examined.The relationship between the expressions of p27,PCNA and ki-67 proteins in synovial sarcoma with its prognosis by clinical stage,tissue grades and prognosis was investigated.Results (1)In normal synovial tissue,the expressions of PCNA(0) and ki-67(0) were positive,p27 were positive100%(10/10).but the expressions of PCNA[86.1%(31/36)]and ki-67[69.4%(25/36)]in most synovial sarcoma were positive,p27 were negative[11.1%(4/36)],significant differences were found among these expressions(χ2=25.16,χ2=9.18,P＜0.05).(2) p27 positive-expression correlated significantly wiht the histological grade of synovial sarcoma(χ2=9.19,P＜0.05)and prognosis(χ2=5.98,P＜0.05).The positive expressions of PCNA and ki-67 were positively related with the clinical stage(χ2=7.40,χ2=8.12,P＜0.05)and tissue grades(χ2=7.17,χ2=9.18,P＜0.05)and prognosis(χ2=13.03,χ2=10.66,P＜0.05)of synovial sarcina(3) There were negative correlations between p27 and PCNA(r=-0.63,P＜0.05) and between p27 and ki-67(r=-0.53,P＜0.05).But positive correlation between PCNA and ki-67(r=0.63,P＞0.05)was found.Conclusions Expressions of PCNA and ki-67 in most synovial sarcomas are positive,and that of p27 is negative,each plays an important role in the proliferation of synovial sarcoma.Combination of measuring of the p27,PCNA and ki-67 is valuble in predicting the prognosis.
