中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2012年
1期
6-10,14
,共6页
徐加英%吉俊敏%焦旸%伍理%樊赛军
徐加英%吉俊敏%焦旸%伍理%樊賽軍
서가영%길준민%초양%오리%번새군
血根碱%卵巢癌%细胞周期%凋亡%放射增敏性
血根堿%卵巢癌%細胞週期%凋亡%放射增敏性
혈근감%란소암%세포주기%조망%방사증민성
Sanguinarine%Ovarian cancer%Cell cycle%Apoptosis%Radiosensitivity
目的 探讨血根碱对卵巢癌SK-OV-3细胞的生长及放射敏感性的影响.方法 采用MTT法和克隆形成法检测血根碱处理后卵巢癌SK-OV-3细胞的生长；流式细胞仪分析方法观察血根碱对细胞周期分布和细胞凋亡的影响；Annexin V/PI法观察血根碱联合辐照对细胞凋亡的影响.结果 0 ～5 μmol/L血根碱处理24和48 h后,细胞生长的抑制与血根碱处理的剂量和时间成正相关(r=0.96和0.97),半数细胞生长抑制的浓度(IC50)分别为3.02和1.11 μmol/L.血根碱处理导致了包括细胞变圆、皱缩,漂浮细胞增多等细胞形态学的改变.Sub-G1凋亡峰随血根碱浓度上升而增高,并出现G0/G1期细胞周期阻滞.0.5μmol/L血根碱预处理24 h后经6 Gy的X射线辐照,早期凋亡细胞从10.28％增加至43.28％(t=19.41,P＜0.01),晚期凋亡细胞从20.26％增加至30.80％(t=8.78,P＜0.01).采用多靶单击模型拟合曲线发现血根碱+X射线组与X射线单独照射组的放射增敏比(SERD0)达1.625,即低浓度的血根碱可以增加卵巢癌细胞的放射敏感性.结论 血根碱可以显著抑制卵巢癌SK-OV-3细胞的生长,诱导细胞凋亡和周期阻滞,而且在低剂量下,血根碱可以增加卵巢癌细胞的放射治疗敏感性.
目的 探討血根堿對卵巢癌SK-OV-3細胞的生長及放射敏感性的影響.方法 採用MTT法和剋隆形成法檢測血根堿處理後卵巢癌SK-OV-3細胞的生長；流式細胞儀分析方法觀察血根堿對細胞週期分佈和細胞凋亡的影響；Annexin V/PI法觀察血根堿聯閤輻照對細胞凋亡的影響.結果 0 ～5 μmol/L血根堿處理24和48 h後,細胞生長的抑製與血根堿處理的劑量和時間成正相關(r=0.96和0.97),半數細胞生長抑製的濃度(IC50)分彆為3.02和1.11 μmol/L.血根堿處理導緻瞭包括細胞變圓、皺縮,漂浮細胞增多等細胞形態學的改變.Sub-G1凋亡峰隨血根堿濃度上升而增高,併齣現G0/G1期細胞週期阻滯.0.5μmol/L血根堿預處理24 h後經6 Gy的X射線輻照,早期凋亡細胞從10.28％增加至43.28％(t=19.41,P＜0.01),晚期凋亡細胞從20.26％增加至30.80％(t=8.78,P＜0.01).採用多靶單擊模型擬閤麯線髮現血根堿+X射線組與X射線單獨照射組的放射增敏比(SERD0)達1.625,即低濃度的血根堿可以增加卵巢癌細胞的放射敏感性.結論 血根堿可以顯著抑製卵巢癌SK-OV-3細胞的生長,誘導細胞凋亡和週期阻滯,而且在低劑量下,血根堿可以增加卵巢癌細胞的放射治療敏感性.
목적 탐토혈근감대란소암SK-OV-3세포적생장급방사민감성적영향.방법 채용MTT법화극륭형성법검측혈근감처리후란소암SK-OV-3세포적생장；류식세포의분석방법관찰혈근감대세포주기분포화세포조망적영향；Annexin V/PI법관찰혈근감연합복조대세포조망적영향.결과 0 ～5 μmol/L혈근감처리24화48 h후,세포생장적억제여혈근감처리적제량화시간성정상관(r=0.96화0.97),반수세포생장억제적농도(IC50)분별위3.02화1.11 μmol/L.혈근감처리도치료포괄세포변원、추축,표부세포증다등세포형태학적개변.Sub-G1조망봉수혈근감농도상승이증고,병출현G0/G1기세포주기조체.0.5μmol/L혈근감예처리24 h후경6 Gy적X사선복조,조기조망세포종10.28％증가지43.28％(t=19.41,P＜0.01),만기조망세포종20.26％증가지30.80％(t=8.78,P＜0.01).채용다파단격모형의합곡선발현혈근감+X사선조여X사선단독조사조적방사증민비(SERD0)체1.625,즉저농도적혈근감가이증가란소암세포적방사민감성.결론 혈근감가이현저억제란소암SK-OV-3세포적생장,유도세포조망화주기조체,이차재저제량하,혈근감가이증가란소암세포적방사치료민감성.
Objective To study the effect of sanguinarine on the growth and radiosensitivity of ovarian cancer SK-OV-3 cells.Methods Cell growth was determined by MTT and clonogenic assay.Cell cycle analysis was performed by flow cytometry assay.The cell apoptosis was analyzed by Annexin V/PI assay.Results Sanguinarine inhibited SK-OV-3 cell growth in a dose-and time-dependent fashion and its IC50 values were 3.02 and 1.11 μmol/L at 24 and 48 h,respectively. Sanguinarine also significantly triggered a sub-G1 peak,an indicator of apoptosis,and caused a G0/G1 arrest.Furthermore,the cell apoptosis induced by X-irradiation was significantly increased at 6 Gy when the cells were pre-treated with sanguinarine,in which the early apoptotic population increased from 10.28％ to 43.28％ (t =19.41,P ＜0.01 ) and the late apoptotic population increased from 20.26％ to 30.80％ ( t =8.78,P ＜ 0.01 ).The multi-target click model was used to fit survival curves and the SER of sanguinarine treatment approached to 1.625 at the dose of D0. Conclusions Sanguinarine could inhibit SK-OV-3 cell growth by inducing apoptosis and cell cycle arrest and enhance cell radiosensitivity at low doses.
