中华创伤骨科杂志
中華創傷骨科雜誌
중화창상골과잡지
CHINESE JOURNAL OF ORTHOPAEDIC TRAUMA
2011年
4期
353-357
,共5页
前交叉韧带%移植,同种异体%兔%交联
前交扠韌帶%移植,同種異體%兔%交聯
전교차인대%이식,동충이체%토%교련
Anterior cruciate ligament%Transplantation,allogenetic%Rabbits%Cross-linking
目的 探讨利用碳化二亚胺(EDC)交联的方法对同种异体肌腱进行预处理,改善其修复效果.方法 获取兔同种异体跟腱,应用EDC交联并肝素化,应用Ⅱ型胶原酶酶解法检测交联组和未交联组肌腱的降解率并进行比较.应用四氮唑蓝法检测EDC交联(EDC交联组)对细胞增殖率(1、3、7 d)的影响,其结果与生理盐水(阴性对照组)及苯酚(阳性对照组)进行比较.取28只雄性SD大鼠,每组14只,随机分为EDC交联组(埋植EDC交联肌腱)和未交联组(埋植未交联肌腱),分别于术后1、4周取材(每一时间点取7只),行HE染色观察免疫反应并进行炎症反应分级比较.用交联组和未交联组肌腱对兔前交叉韧带损伤进行修复,1、3、6个月取材做光镜和扫描电镜观察,对比不同时间段腱骨愈合情况.结果 EDC交联并肝素化组降解率[(6.26±3.16)%]低于未交联组[(30.70±10.24)%],差异有统计学意义(t=14.200,P=0.025).培养1、3、7 d.阴性对照组和EDC交联组的相对增殖度分别与阳性对照组比较,差异均有统计学意义(P＜0.05,表1),EDC交联组肌腱无明显细胞毒性作用;1、4周未交联组和交联组炎症反应分级,差异有统计学意义(P＜0.05).移植后组织学和电镜扫描观察可见未交联组愈合比交联组慢.结论 EDC交联并肝素化的肌腱,其稳定性增强,抗酶降解能力提高,免疫原性降低,且腱骨愈合加快.
目的 探討利用碳化二亞胺(EDC)交聯的方法對同種異體肌腱進行預處理,改善其脩複效果.方法 穫取兔同種異體跟腱,應用EDC交聯併肝素化,應用Ⅱ型膠原酶酶解法檢測交聯組和未交聯組肌腱的降解率併進行比較.應用四氮唑藍法檢測EDC交聯(EDC交聯組)對細胞增殖率(1、3、7 d)的影響,其結果與生理鹽水(陰性對照組)及苯酚(暘性對照組)進行比較.取28隻雄性SD大鼠,每組14隻,隨機分為EDC交聯組(埋植EDC交聯肌腱)和未交聯組(埋植未交聯肌腱),分彆于術後1、4週取材(每一時間點取7隻),行HE染色觀察免疫反應併進行炎癥反應分級比較.用交聯組和未交聯組肌腱對兔前交扠韌帶損傷進行脩複,1、3、6箇月取材做光鏡和掃描電鏡觀察,對比不同時間段腱骨愈閤情況.結果 EDC交聯併肝素化組降解率[(6.26±3.16)%]低于未交聯組[(30.70±10.24)%],差異有統計學意義(t=14.200,P=0.025).培養1、3、7 d.陰性對照組和EDC交聯組的相對增殖度分彆與暘性對照組比較,差異均有統計學意義(P＜0.05,錶1),EDC交聯組肌腱無明顯細胞毒性作用;1、4週未交聯組和交聯組炎癥反應分級,差異有統計學意義(P＜0.05).移植後組織學和電鏡掃描觀察可見未交聯組愈閤比交聯組慢.結論 EDC交聯併肝素化的肌腱,其穩定性增彊,抗酶降解能力提高,免疫原性降低,且腱骨愈閤加快.
목적 탐토이용탄화이아알(EDC)교련적방법대동충이체기건진행예처리,개선기수복효과.방법 획취토동충이체근건,응용EDC교련병간소화,응용Ⅱ형효원매매해법검측교련조화미교련조기건적강해솔병진행비교.응용사담서람법검측EDC교련(EDC교련조)대세포증식솔(1、3、7 d)적영향,기결과여생리염수(음성대조조)급분분(양성대조조)진행비교.취28지웅성SD대서,매조14지,수궤분위EDC교련조(매식EDC교련기건)화미교련조(매식미교련기건),분별우술후1、4주취재(매일시간점취7지),행HE염색관찰면역반응병진행염증반응분급비교.용교련조화미교련조기건대토전교차인대손상진행수복,1、3、6개월취재주광경화소묘전경관찰,대비불동시간단건골유합정황.결과 EDC교련병간소화조강해솔[(6.26±3.16)%]저우미교련조[(30.70±10.24)%],차이유통계학의의(t=14.200,P=0.025).배양1、3、7 d.음성대조조화EDC교련조적상대증식도분별여양성대조조비교,차이균유통계학의의(P＜0.05,표1),EDC교련조기건무명현세포독성작용;1、4주미교련조화교련조염증반응분급,차이유통계학의의(P＜0.05).이식후조직학화전경소묘관찰가견미교련조유합비교련조만.결론 EDC교련병간소화적기건,기은정성증강,항매강해능력제고,면역원성강저,차건골유합가쾌.
Objective To explore a new way to improve the repairing effect of tendon allograft by pretreatment with ethyldimethylaminopropyl carbodiimide (EDC) cross-linking. Methods Rabbits' tendon allografts were obtained by lyopyilization.The allografts were then cross-linked and heparinized by EDC and N-hydroxy-succinimide (NHS) respectively.The degradation rate,cytotoxicity and histocompatibility of the cross-linked allografts were detected and compared with those of untreated at different time points.The injured anterior cruciate ligaments (ACL) were repaired by tendon allografts with and without cross-linking pretreatment in rabbits.The tendon-bone healing was observed and compared by light microscopy and scanning electron microscopy (SEM) at 1,3,6 months. Results The degradation rate of the pretreated tendon allografts [(6.26 ± 3.16)％] was significantly lower than that of the unpretreated [(30.70 ± 10.24)％]( t = 14.200,P = 0.025 ).The pretreated tendon allografts were atoxigenic and produced significantly lower inflammatory reaction ( P ＜ 0.05 ).The pretreated tendon allografts also showed more powerful capability of repairing ACL injury and shorter time of tendon-bone healing. Conclusion Tendon allografts cross-linked and heparinized by EDC/NHS may promote tendon-bone healing with improved stability,degradation and biocompatibility.
