CAJ | 학술논문

目的制备一种载染料苏丹黑的聚乳酸羟基乙酸高分子超声造影剂(SB-PLGA),研究其增强兔肿瘤淋巴结显像的效果及作为前哨淋巴结(SLN)活检指示剂的可行性和价值.方法采用双乳化法和冷冻干燥法制备载苏丹黑的高分子超声造影剂.建立兔大腿皮下VX2肿瘤淋巴结模型8只,经足垫皮下注射造影剂,超声造影显像胭窝肿瘤前哨淋巴结及第二站腹股沟淋巴结,观察其造影增强情况.分别于30 min后行腘窝淋巴结清扫切除蓝染的淋巴结,观察第一站腘窝淋巴结及第二站腹股沟淋巴结的染色情况,并行冰冻切片HE染色.结果 SB-PLGA超声造影剂形态规则,粒径分布均匀；超声造影可明显增强肿瘤淋巴结显像,而第二站淋巴结显像不明显；胭窝淋巴结清扫术中可见淋巴结染色效果好,30 min后,冰冻切片HE染色可见淋巴结内造影剂存在于淋巴窦内,并有大量进入巨噬细胞；行腹股沟淋巴结清扫,均未见第二站淋巴结蓝染.结论 SB-PLGA超声造影剂是一种良好的肿瘤淋巴结显影剂和指示剂.淋巴结内巨噬细胞吞噬造影剂后导致造影剂在淋巴结内滞留和聚集可能与其增强淋巴结显影的机制有关.
목적 제비일충재염료소단흑적취유산간기을산고분자초성조영제(SB-PLGA),연구기증강토종류림파결현상적효과급작위전초림파결(SLN)활검지시제적가행성화개치.방법 채용쌍유화법화냉동간조법제비재소단흑적고분자초성조영제.건립토대퇴피하VX2종류림파결모형8지,경족점피하주사조영제,초성조영현상연와종류전초림파결급제이참복고구림파결,관찰기조영증강정황.분별우30 min후행객와림파결청소절제람염적림파결,관찰제일참객와림파결급제이참복고구림파결적염색정황,병행빙동절편HE염색.결과 SB-PLGA초성조영제형태규칙,립경분포균균；초성조영가명현증강종류림파결현상,이제이참림파결현상불명현；연와림파결청소술중가견림파결염색효과호,30 min후,빙동절편HE염색가견림파결내조영제존재우림파두내,병유대량진입거서세포；행복고구림파결청소,균미견제이참림파결람염.결론 SB-PLGA초성조영제시일충량호적종류림파결현영제화지시제.림파결내거서세포탄서조영제후도치조영제재림파결내체류화취집가능여기증강림파결현영적궤제유관.
Objective To prepare polyclactic-co-glycolic acid(PLGA) polymer ultrasound contrast agent loading sudan black dye (SB-PLGA) and study the enhancement effect of it on rabbit lymph nodes imaging and as a sentinel lymph node (SLN) biopsy in the feasibility and value of the indicator.Methods SB-PLGA ultrasound contrast agent was made by double emulsion and freeze-drying methods.Thigh subcutaneous rabbit VX2 tumor model of eight lymph nodes were set up with the foot pad subcutaneous injection of contrast agents to get contrast-enhanced ultrasound imaging of the popliteal lymph node and the second inguinal lymph node stations.Then the blue stained lymph nodes were resected by popliteal lymph node dissection and the second station lymph nodes were also observed after 30 mins respectively,lymph nodes were examined by frozen sections HE staining in parallel.Results SB-PLGA microbubbles had a tight size distribution.Ultrasound imaging can be significantly enhanced lymph node imaging,the second station lymph node imaging was not obvious.Popliteal lymph node dissection for lymph node staining,30minutes later,frozen sections HE staining were seen within the lymph node contrast agent present in the lymphatic sinus and a large entry of macrophages.Inguinal lymph node dissection showed the second station of lymph nodes no blue staining.Conclusions SB-PLGA ultrasound contrast agent is good for sentinel lymph node imaging and biopsy indicator.Contrast agent retention and accumulation in the lymph nodes by the uptake of macrophages may be associated with the mechanisms of lymph node enhanced imaging.