中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2012年
5期
449-454
,共6页
李锦波%陈淑琴%袁力%姜红叶
李錦波%陳淑琴%袁力%薑紅葉
리금파%진숙금%원력%강홍협
子宫腺肌症%子宫肌瘤%人β防御素2%异位内膜%基因表达
子宮腺肌癥%子宮肌瘤%人β防禦素2%異位內膜%基因錶達
자궁선기증%자궁기류%인β방어소2%이위내막%기인표체
Adenomyosis%Hysteromyoma%Human β defensin-2%Ectopic endometrium%Gene expression
目的 检测人β防御素2(hBD-2)在正常子宫内膜和子宫腺肌症(AM)患者的在位和异位内膜组织中的基因和蛋白表达.方法 选择AM和非子宫内膜异位症(EMS)的子宫肌瘤患者各25例,分别取AM异位病灶组织(AM异位内膜组)和相应的在位子宫内膜组织(AM在位内膜组)以及子宫肌瘤患者的子宫内膜组织(对照组).采用实时荧光定量PCR检测各组内膜组织中hBD-2和白细胞介素-1β(IL-1β)、白细胞介素石(IL-6)、肿瘤坏死因子α(TNF-α)等的基因表达及其相关性,并免疫组化检测其蛋白的表达.结果 将各组的hBD-2、IL-1β、IL-6和TNF-α的基因表达进行比较,AM异位内膜组与AM在位内膜组、AM在位内膜组与对照组之间差异均无统计学意义(P均＞0.05).AM异位内膜组hBD-2和IL-1β的基因表达分别为0.0320(0.0095 ～0.0690)和0.0427(0.0038 ～0.0975),对照组分别为0.0034(0.0025～0.0424)和0.0080(0.0040 ～0.0251),前者均明显高于后者(P值分别为0.032和0.025).AM异位内膜组hBD-2的基因表达与该组的TNF-α的基因表达呈正相关(r=0.857,P=0.014),与IL-1β、IL-6的基因表达无相关性(r值分别为0.750、0.464,P值分别为0.052、0.464).结论 AM的形成可能与异位病灶组织中的hBD-2的表达无明显关系,这可能与AM患者异位病灶组织中炎症因子无明显上调有关.
目的 檢測人β防禦素2(hBD-2)在正常子宮內膜和子宮腺肌癥(AM)患者的在位和異位內膜組織中的基因和蛋白錶達.方法 選擇AM和非子宮內膜異位癥(EMS)的子宮肌瘤患者各25例,分彆取AM異位病竈組織(AM異位內膜組)和相應的在位子宮內膜組織(AM在位內膜組)以及子宮肌瘤患者的子宮內膜組織(對照組).採用實時熒光定量PCR檢測各組內膜組織中hBD-2和白細胞介素-1β(IL-1β)、白細胞介素石(IL-6)、腫瘤壞死因子α(TNF-α)等的基因錶達及其相關性,併免疫組化檢測其蛋白的錶達.結果 將各組的hBD-2、IL-1β、IL-6和TNF-α的基因錶達進行比較,AM異位內膜組與AM在位內膜組、AM在位內膜組與對照組之間差異均無統計學意義(P均＞0.05).AM異位內膜組hBD-2和IL-1β的基因錶達分彆為0.0320(0.0095 ～0.0690)和0.0427(0.0038 ～0.0975),對照組分彆為0.0034(0.0025～0.0424)和0.0080(0.0040 ～0.0251),前者均明顯高于後者(P值分彆為0.032和0.025).AM異位內膜組hBD-2的基因錶達與該組的TNF-α的基因錶達呈正相關(r=0.857,P=0.014),與IL-1β、IL-6的基因錶達無相關性(r值分彆為0.750、0.464,P值分彆為0.052、0.464).結論 AM的形成可能與異位病竈組織中的hBD-2的錶達無明顯關繫,這可能與AM患者異位病竈組織中炎癥因子無明顯上調有關.
목적 검측인β방어소2(hBD-2)재정상자궁내막화자궁선기증(AM)환자적재위화이위내막조직중적기인화단백표체.방법 선택AM화비자궁내막이위증(EMS)적자궁기류환자각25례,분별취AM이위병조조직(AM이위내막조)화상응적재위자궁내막조직(AM재위내막조)이급자궁기류환자적자궁내막조직(대조조).채용실시형광정량PCR검측각조내막조직중hBD-2화백세포개소-1β(IL-1β)、백세포개소석(IL-6)、종류배사인자α(TNF-α)등적기인표체급기상관성,병면역조화검측기단백적표체.결과 장각조적hBD-2、IL-1β、IL-6화TNF-α적기인표체진행비교,AM이위내막조여AM재위내막조、AM재위내막조여대조조지간차이균무통계학의의(P균＞0.05).AM이위내막조hBD-2화IL-1β적기인표체분별위0.0320(0.0095 ～0.0690)화0.0427(0.0038 ～0.0975),대조조분별위0.0034(0.0025～0.0424)화0.0080(0.0040 ～0.0251),전자균명현고우후자(P치분별위0.032화0.025).AM이위내막조hBD-2적기인표체여해조적TNF-α적기인표체정정상관(r=0.857,P=0.014),여IL-1β、IL-6적기인표체무상관성(r치분별위0.750、0.464,P치분별위0.052、0.464).결론 AM적형성가능여이위병조조직중적hBD-2적표체무명현관계,저가능여AM환자이위병조조직중염증인자무명현상조유관.
Objective To investigate the expression of human β defensin-2(hβD-2)in the eutopic and ectopic endometrial tissue in patients with adenomyosis and in women with normal endometrium.Methods Twenty-five hysteromyoma patients with adenomyosis(AM) and 25 hysteromyoma patients without endometriosis (EMS) were selected and divided into three groups:AM ectopic endometrium group,AM eutopic endometrium group and control group( endometrial tissue in patients with hysteromyoma).The level of mRNA expressions of hβD-2,interleukin-1β ( IL-1β ),interleukin-6 ( IL-6 ) and tumor necrosis factor-α (TNF-α) was investigated quantitatively using Real Time PCR (RT-PCR) and the corresponding protein level was detected by immunohistochemistry.Results Comparing the expression of hβD-2,IL-1β,IL-6,TNF-α genes among the three groups,there was no significant difference between ectopic endometrium group and eutopic endometrium group and there was also no significant difference between eutopic endometrium group and the control group.( P ＞ 0.05 ).The expression of hβD-2 and IL-1β were 0.0320 (0.0095 ～ 0.0690 ) and 0.0427 ( 0.0038 ～ 0.0975 ) in the ectopic endometrium group,and they were 0.0034(0.0025 ～0.0424) and 0.0080(0.0040 ～0.0251 ) in the control group,respectively.They were both significantly higher in ectopic endometrium group than in the control group (P ＜ 0.05 ).In the ectopic endometrium group hβD-2 expression was positively correlated with the level of TNF-α ( r =0.857,P =0.014 ),and it had no correlation with both IL-1β and IL-6 ( r =0.750,P =0.052 ; r =0.464,P =0.464; respectively)Conclusion HβD-2 might not play an important role in the formation of adenomyosis.It may be related to no significant up-regulation of inflammatory factors in ectopic lesion tissue.
