CAJ | 학술논문

目的研究不同转移潜能人肝癌细胞系及其肝癌组织中骨桥蛋白(OPN)表达情况,分析OPN表达与肝癌细胞侵袭转移潜能、肝癌术后复发转移的关系.方法应用细胞免疫组化、半定量RT-PCR、Western Blot和ELISA检测不同转移潜能人肝癌细胞系SMMC-7721、MHCC97-L和HCCLM6 OPN表达情况.应用组织芯片、免疫组化检测200例肝癌组织中OPN表达.结果 OPN表达水平随着肝癌细胞侵袭转移潜能增加逐渐升高.肝癌组织中OPN表达与病人性别、年龄、血清AFP水平、肿瘤大小、有无包膜、细胞分化以及肝门淋巴结转移无明显相关性.而与肝癌TNM分期、门静脉癌栓和术后复发转移发生有明显相关(P<0.05).术后有复发转移病人的肝癌组织中66%表达OPN,而术后无复发转移病人的肝癌组织中仅37%表达OPN,两者比较差异具有显著性(P<0.05).结论 OPN表达与肝癌细胞细胞系侵袭转移潜能密切相关.肝癌组织中OPN表达可能是预测肝癌术后是否发生复发转移指标.
목적 연구불동전이잠능인간암세포계급기간암조직중골교단백(OPN)표체정황,분석OPN표체여간암세포침습전이잠능、간암술후복발전이적관계.방법 응용세포면역조화、반정량RT-PCR、Western Blot화ELISA검측불동전이잠능인간암세포계SMMC-7721、MHCC97-L화HCCLM6 OPN표체정황.응용조직심편、면역조화검측200례간암조직중OPN표체.결과 OPN표체수평수착간암세포침습전이잠능증가축점승고.간암조직중OPN표체여병인성별、년령、혈청AFP수평、종류대소、유무포막、세포분화이급간문림파결전이무명현상관성.이여간암TNM분기、문정맥암전화술후복발전이발생유명현상관(P<0.05).술후유복발전이병인적간암조직중66%표체OPN,이술후무복발전이병인적간암조직중부37%표체OPN,량자비교차이구유현저성(P<0.05).결론 OPN표체여간암세포세포계침습전이잠능밀절상관.간암조직중OPN표체가능시예측간암술후시부발생복발전이지표.
Objective To study the relationship between OPN expression and HCC eellline me-tastatic potentiaI and HCC recurrence/metastasis after resection.Methods Immunocytochemistry,RT-PCR,Western Blot and ELISA were applied to detect OPN expression of HCC cell lines SMMC-7721,MHCC97-L and HCCLM6 with different metastastic potential.Immunohistochemistry of OPN was performed in the tissue arrays containing 200 HCC samples.Results The OPN expression was leveled with increasing metastatic potential of celIlines.OPN expression of samples was not associated with age.sex,serum AFP level,tumor size,cell differentiation,tumor capsule and lymphatic node metastasis but significantly associated with HCC TN M classification.portal vein tumor thrombi and HCC recurrence/metastasis after resection.OPN expression detected in 66% of HCC samples of pa-tients with recurrence/metastasis after resection was significantly higher than 37% of the samples of pa-dents without recurrence/metastasis after resection(P<0.05).Conclusion OPN expression iS associated with HCC cell line metastatic potential and maybe an indicator of HCC recurrence/metastasis after resection.