CAJ | 학술논문

目的探讨Abelson非受体酪氨酸激酶(c-Abl)及肌动蛋白骨架结构与主动脉夹层的关系.方法夹层主动脉标本获取白行A型主动脉夹层手术的患者,分为高血压组(n=9)和正常血压组(n=15),对照组来自移植心脏的主动脉标本(n=8).bax蛋白与bcl-2蛋白比值评估凋亡水平.纤维化肌动蛋白(F-actin)和球状单体肌动蛋白(G-actin)含量比值分析肌动蛋白细胞骨架动态结构.免疫印迹分析测量c-Abl和磷酸化c-Abl蛋白水平.结果 bax蛋白与bcl-2蛋白比值在正常对照组,高血压夹层组和正常血压夹层组内分别为(0.97±0.02、2.45±0.06、2.30±0.12),夹层组中比值升高(P＜0.01).F-actin与G-actin含量比值分别为(1.53±0.03、3.00±0.03、2.72±0.04),夹层组中比值升高(P＜0.01).C-Abl蛋白含量比值分别为(0.12±0.01、0.36±0.01、0.41±0.02),夹层组中含量增加(P＜0.01)；磷酸化c-Abl蛋白含量比值分别为(0.0047±0.0003、0.023±0.001、0.021±0.001),夹层组中含量增加(P＜0.01).上述数据在高血压组与正常血压组比较差异无统计学意义(P＞0.05).结论 c-Abl及其激活与主动脉夹层的相关性.C-Abl及其激活可能通过调节血管平滑肌细胞的凋亡和肌动蛋白细胞骨架结构来参与主动脉夹层的发生和发展.
목적 탐토Abelson비수체락안산격매(c-Abl)급기동단백골가결구여주동맥협층적관계.방법 협층주동맥표본획취백행A형주동맥협층수술적환자,분위고혈압조(n=9)화정상혈압조(n=15),대조조래자이식심장적주동맥표본(n=8).bax단백여bcl-2단백비치평고조망수평.섬유화기동단백(F-actin)화구상단체기동단백(G-actin)함량비치분석기동단백세포골가동태결구.면역인적분석측량c-Abl화린산화c-Abl단백수평.결과 bax단백여bcl-2단백비치재정상대조조,고혈압협층조화정상혈압협층조내분별위(0.97±0.02、2.45±0.06、2.30±0.12),협층조중비치승고(P＜0.01).F-actin여G-actin함량비치분별위(1.53±0.03、3.00±0.03、2.72±0.04),협층조중비치승고(P＜0.01).C-Abl단백함량비치분별위(0.12±0.01、0.36±0.01、0.41±0.02),협층조중함량증가(P＜0.01)；린산화c-Abl단백함량비치분별위(0.0047±0.0003、0.023±0.001、0.021±0.001),협층조중함량증가(P＜0.01).상술수거재고혈압조여정상혈압조비교차이무통계학의의(P＞0.05).결론 c-Abl급기격활여주동맥협층적상관성.C-Abl급기격활가능통과조절혈관평활기세포적조망화기동단백세포골가결구래삼여주동맥협층적발생화발전.
Objective To determine the possible relationship between abelson non-receptor tyrosine kinase (c-Abl) and aortic dissection.Methods Aortic specimens were obtained from patients undergoing aortic replacement surgery for type A aortic dissection with high blood pressure ( n =9,HBP-AD group) and with normal blood pressure (n =15,NBP-AD group),along with control donors dying of causes unrelated to aortic disease (n =8,cpmtrol group,collected during heart transplantation).Apoptosis was also assessed by bax/bcl-2.Filamentous actin (F-actin) and global actin (G-actin) were tested as a measure of actin cytoskeleton dynamics.Western blotting was performed to evaluate c-Abl and phosphorylated c-Abl protein levels.Results bax/B lymphocytes/leukemia-2 (bcl-2) were elevated in aortic tissue from patients with aortic dissection as compared with those in controls (Controls:0.97 ±0.02; HBP-AD:2.45 ± 0.06,P ＜ 0.01 vs controls; NBP-AD:2.30 ± 0.12,P ＜ 0.01 vs Controls).Actin polymerization was increased in aortic tissue ( Controls:1.53 ± 0.03; HBP-AD:3.00 ± 0.03,P ＜ 0.01 vs Controls;NBP-AD:2.72 ± 0.04,P ＜ 0.01 vs Controls).C-Abl ( Controls:0.12 ± 0.01 ; HBP-AD:0.36 ± 0.01,P ＜0.01 vs Controls; NBP-AD:0.41 ±0.02,P ＜0.01 vs Controls) and phosphorylated c-Abl protein levels ( Controls:0.0047 ±0.0003 ; HBP-AD:0.0230 ±0.0010,P ＜0.01 vs Controls; NBP-AD:0.0210 ±0.0010,P＜0.01 vs Controls) wer also elevated.However,there was no significant difference between HBP-AD group and NBP-AD group in all data ( P ＞ 0.05 ).Conclusion c-Abl is associated with aortic dissection,probably by regulating apoptosis and the actin cytoskeleton.c-Abl could be a potential target of prevention and treatment for aortic dissection.