CAJ | 학술논문

目的:观察针灸对克罗恩病大鼠血清TNF-a、sTNFR-Ⅰ、sTNFR-Ⅱ的影响.方法:采用TNBS制备大鼠克罗恩病模型,随机分为模型组、隔药灸组、电针组,并与正常大鼠作对照,采用HE染色观察结肠黏膜组织病理学变化,应用ELISA方法检测血清TNF-a、sTNFR-Ⅰ、sTNFR-Ⅱ的含量.结果:与正常组比较,CD大鼠血清YNF-a显著升高、sTNFR-Ⅰ和sTNR-Ⅱ无显著变化.经隔药灸和电针治疗后血清TNF-a水平显著降低,sTNFR-Ⅰ和sTNFR-Ⅱ无显著变化.CD大鼠结肠组织的炎症病变和异常的组织结构在隔药灸、电针治疗后得到明显改善.结论:隔药灸与电针均能够显著降低血清TNF-a的含量,该作用可能是隔药灸与电针治疗CD的作用机制之一.
목적:관찰침구대극라은병대서혈청TNF-a、sTNFR-Ⅰ、sTNFR-Ⅱ적영향.방법:채용TNBS제비대서극라은병모형,수궤분위모형조、격약구조、전침조,병여정상대서작대조,채용HE염색관찰결장점막조직병이학변화,응용ELISA방법검측혈청TNF-a、sTNFR-Ⅰ、sTNFR-Ⅱ적함량.결과:여정상조비교,CD대서혈청YNF-a현저승고、sTNFR-Ⅰ화sTNR-Ⅱ무현저변화.경격약구화전침치료후혈청TNF-a수평현저강저,sTNFR-Ⅰ화sTNFR-Ⅱ무현저변화.CD대서결장조직적염증병변화이상적조직결구재격약구、전침치료후득도명현개선.결론:격약구여전침균능구현저강저혈청TNF-a적함량,해작용가능시격약구여전침치료CD적작용궤제지일.
Objeetive:To observe the effect of acupuncture-moxibustion on TNF-a,sTNFR-Ⅰand sTNFR-Ⅱ in rats with Crohn's disease(CD).Method:The models of CD rats induced by TNBS were randomized into model group.herbal cake-partitioned moxibustion group and electroacupuncture group as well as a normal control group.The histopathological changes of colon mucus membrane were observed with HE staining and the contents Of TNF-a.sTNFR-Ⅰand sTNFR-Ⅱ were detected with ELISA method.Results:When compared with the normal group,the TNF-a level in CD rats was substantially elevated and the sTNFR-Ⅰ and sTNFR-Ⅱ showed no marked changes.After herbal cake-partitioned moxibustion and electroacupuncture treatment,the TNF-a level in CD rats was substantially reduced and sTNFRL-Ⅰ and sTNFR-Ⅱ showed no marked changes.The inflammatory lesions and abnormal structures of CD rats were significantly improved after herbal cake-partitioned moxibustion and electric stimulation.Conclusion:Herbal cake-partitioned moxibustion and electroacupuncture Can both remarkably reduce the TNF-a level in blood serum.and this might be one ofthe action mechanisms in the treatment of CD.