化学与生物工程
化學與生物工程
화학여생물공정
CHEMISTRY & BIOENGINEERING
2009年
7期
64-66
,共3页
王伟平%李伟%邱雁临%张华山%彭晓斌
王偉平%李偉%邱雁臨%張華山%彭曉斌
왕위평%리위%구안림%장화산%팽효빈
石油污染%紫外分光光度法%石油降解菌%降解率
石油汙染%紫外分光光度法%石油降解菌%降解率
석유오염%자외분광광도법%석유강해균%강해솔
oil pollution%UV spectrophotometry%oil-degrading microorganism%degradation rate
经过以石油烃为唯一碳源的选择性培养基平板初筛和三角瓶发酵复筛,采用紫外分光光度法测定石油降解率,从江汉油田和冀东油田石油污染的土壤和水体中,筛选出有降解石油能力的微生物9株.其中3株细菌(X-1,X-2,X-3)降解石油能力较高,X-1菌株的石油降解率最高达64.28%.根据形态学观察和部分生理生化特征初步鉴定,该菌为节杆菌属(Arthrobacter sp.).
經過以石油烴為唯一碳源的選擇性培養基平闆初篩和三角瓶髮酵複篩,採用紫外分光光度法測定石油降解率,從江漢油田和冀東油田石油汙染的土壤和水體中,篩選齣有降解石油能力的微生物9株.其中3株細菌(X-1,X-2,X-3)降解石油能力較高,X-1菌株的石油降解率最高達64.28%.根據形態學觀察和部分生理生化特徵初步鑒定,該菌為節桿菌屬(Arthrobacter sp.).
경과이석유경위유일탄원적선택성배양기평판초사화삼각병발효복사,채용자외분광광도법측정석유강해솔,종강한유전화기동유전석유오염적토양화수체중,사선출유강해석유능력적미생물9주.기중3주세균(X-1,X-2,X-3)강해석유능력교고,X-1균주적석유강해솔최고체64.28%.근거형태학관찰화부분생리생화특정초보감정,해균위절간균속(Arthrobacter sp.).
Nine strains which could degrade crude oil efficiently were isolated from oil-containing soil or water of Jianghan Oil Field and Jidong Oil Field,and were screened on the plates and flasks respectively in the selective medium with oil as sole carbon source. The results showed that three bacteria (X-1,X-2,X-3) had relatively high capacity of oil degradation and the best one was strain X-1,with degradation rate of oil as high as 64.28%.It was belong to Arthrobacter sp. by preliminary identification through configuration observation and physiological and biochemical characteristics.