CAJ | 학술논문

对赤灵芝原生质体制备、再生及紫外诱变育种、筛选有机锗高产菌株进行研究.结果表明:赤灵芝原生质体制备与再生的最佳条件为菌龄96h,复合酶液浓度为2%溶壁酶、1%蜗牛酶和1%纤维素酶,温度31℃,时间2h,获得赤灵芝原生质体产量为4.33×106个/ml,再生率达0.00275%.原生质体最佳紫外诱变条件为:15W紫外灯,距离30cm,照射30s,致死率为66.7%.最终选育出3株高产有机锗诱变株,有机锗含量分别比出发菌株提高了113.58%、105.87%、103.86%.
대적령지원생질체제비、재생급자외유변육충、사선유궤타고산균주진행연구.결과표명:적령지원생질체제비여재생적최가조건위균령96h,복합매액농도위2%용벽매、1%와우매화1%섬유소매,온도31℃,시간2h,획득적령지원생질체산량위4.33×106개/ml,재생솔체0.00275%.원생질체최가자외유변조건위:15W자외등,거리30cm,조사30s,치사솔위66.7%.최종선육출3주고산유궤타유변주,유궤타함량분별비출발균주제고료113.58%、105.87%、103.86%.
Preparation, regeneration, UV mutagenization of Ganoderma lucidum protoplast were studied to obtain high-yield strains producing organogemanium. Under optimized conditions for the preparation and regeneration of Ganoderma lucidum protoplast including cell age 96 h, lywallzyme concentration 2%, glusulase concentration 1%, cellulase concentration 1%, and temperature 31 "Cfor an enzymolysis duration of 2 h, the protoplast yield and regeneration rate were found to be 4.33 × 106 protoplasts/ml and 0.00275%, respectively. When Ganoderma lucidum protoplast was in-adiated for 30 s with 15 W ultraviolet lamp 30 cm away flora it, the lethality was 66.7 % and three high-yield strains were obtained after two passages, the organogemanium contents in whose dried mycelia were higher than that of starting strain by 113.58%, 105.87% and 103.86% separately.