CAJ | 학술논문

以6种草坪草种子和2种草坪草愈伤组织为材料,研究了基本培养基种类和2,4-D浓度对愈伤组织诱导以及不同的细胞分裂素对分化的影响.试验结果表明,N_6培养基较MS、NB培养基适合草坪草组织培养,诱导愈伤的2,4-D浓度宜采用6 mg/L,有机添加物对愈伤组织的生长有利,特别是肌醇对草坪草愈伤组织的生长有明显的促进作用.在分化培养中,不同材料所需的细胞分裂素适宜种类需要通过试验来确定.
이6충초평초충자화2충초평초유상조직위재료,연구료기본배양기충류화2,4-D농도대유상조직유도이급불동적세포분렬소대분화적영향.시험결과표명,N_6배양기교MS、NB배양기괄합초평초조직배양,유도유상적2,4-D농도의채용6 mg/L,유궤첨가물대유상조직적생장유리,특별시기순대초평초유상조직적생장유명현적촉진작용.재분화배양중,불동재료소수적세포분렬소괄의충류수요통과시험래학정.
The effects of some media and concentration of 2,4-D on callus-induction with 6 kinds of Turf-grass seeds and 2 kinds of Turfgrass calli,and 3 sorts of cytokinins on callus differentiation were studied. The results showed that N_6 is the optimal medium in all of the three media tested and the proper concentration of 2,4-D is 6 mg/L. The organic supplements used in subculture media, especially inositol, benefited callus growth. In differentiation culture,the suitable kinds of cytokinins needed for different materials must be decided by trial.