时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2010年
2期
388-390
,共3页
马齿苋%甜菜红素%抗肿瘤%环磷酰胺%S_(180)小鼠
馬齒莧%甜菜紅素%抗腫瘤%環燐酰胺%S_(180)小鼠
마치현%첨채홍소%항종류%배린선알%S_(180)소서
Portulaca oleracea L.%Betacyanins%Anti-tumor%Cyclophosphamide%S_(180) mice
目的 观察马齿苋甜菜红素联合环磷酰胺(CTX)的抗肿瘤作用.方法 将接种S_(180)瘤细胞的小鼠分为荷瘤对照组、CTX组、马齿苋甜菜红素(高、中、低剂量)+CTX组.给药10 d,进行抑瘤率计算,以及外周血常规、骨髓有核细胞计数、脾脏、胸腺指数、免疫球蛋白、肝肾功能等的检测.结果 与荷瘤对照组相比,CTX组肿瘤生长明显受到抑制.马齿苋甜菜红素低、中、高剂量联合CTX各组抑瘤率明显高于CTX组的抑瘤率.与荷瘤对照组相比,单一CTX处理组脾脏和胸腺指数、IgA、IgG、IgM水平明显降低,WBC、PLT和骨髓有核细胞计数降低,ALT、BUN升高.不同剂量的马齿苋甜菜红素联合CTX处理,可以不同程度地改善脾脏和胸腺指数、IgG、IgM等免疫水平指标,提高WBC、PLT和骨髓有核细胞的数量,降低ALT和BUN.结论 马齿苋甜菜红素对小鼠S_(180)肉瘤有抑制作用,对化疗药物CTX抗S_(180)小鼠具有增效减毒的作用.
目的 觀察馬齒莧甜菜紅素聯閤環燐酰胺(CTX)的抗腫瘤作用.方法 將接種S_(180)瘤細胞的小鼠分為荷瘤對照組、CTX組、馬齒莧甜菜紅素(高、中、低劑量)+CTX組.給藥10 d,進行抑瘤率計算,以及外週血常規、骨髓有覈細胞計數、脾髒、胸腺指數、免疫毬蛋白、肝腎功能等的檢測.結果 與荷瘤對照組相比,CTX組腫瘤生長明顯受到抑製.馬齒莧甜菜紅素低、中、高劑量聯閤CTX各組抑瘤率明顯高于CTX組的抑瘤率.與荷瘤對照組相比,單一CTX處理組脾髒和胸腺指數、IgA、IgG、IgM水平明顯降低,WBC、PLT和骨髓有覈細胞計數降低,ALT、BUN升高.不同劑量的馬齒莧甜菜紅素聯閤CTX處理,可以不同程度地改善脾髒和胸腺指數、IgG、IgM等免疫水平指標,提高WBC、PLT和骨髓有覈細胞的數量,降低ALT和BUN.結論 馬齒莧甜菜紅素對小鼠S_(180)肉瘤有抑製作用,對化療藥物CTX抗S_(180)小鼠具有增效減毒的作用.
목적 관찰마치현첨채홍소연합배린선알(CTX)적항종류작용.방법 장접충S_(180)류세포적소서분위하류대조조、CTX조、마치현첨채홍소(고、중、저제량)+CTX조.급약10 d,진행억류솔계산,이급외주혈상규、골수유핵세포계수、비장、흉선지수、면역구단백、간신공능등적검측.결과 여하류대조조상비,CTX조종류생장명현수도억제.마치현첨채홍소저、중、고제량연합CTX각조억류솔명현고우CTX조적억류솔.여하류대조조상비,단일CTX처리조비장화흉선지수、IgA、IgG、IgM수평명현강저,WBC、PLT화골수유핵세포계수강저,ALT、BUN승고.불동제량적마치현첨채홍소연합CTX처리,가이불동정도지개선비장화흉선지수、IgG、IgM등면역수평지표,제고WBC、PLT화골수유핵세포적수량,강저ALT화BUN.결론 마치현첨채홍소대소서S_(180)육류유억제작용,대화료약물CTX항S_(180)소서구유증효감독적작용.
Objective To explore the role of betacyanins extracted from Portulaca oleracea L. in the therapeutic effect of cyclophosphamide (CTX) and its side-effect in mice loaded with S_(180) murine sarcoma cells.Methods The mice inoculated with S180 murine sarcoma cells were divided into 5 groups: S_(180) control group, sc 2 mg/kg·d of CTX group, ig betacyanins (250,500 and1000 mg/ kg·d ) + sc CTX groups. After 10-day treatment, the tumor inhibition rate, peripheral blood cells, bone marrow karyocytes, immunoglobulin (Ig), the index of spleen and thymus, the liver and renal function were evaluated respectively. Results Although CTX application separately led to decrease of the indexes of spleen and thymus and the levels of IgG and IgM, the CTX application combined with different doses of betacyanin not only increased the tumor inhibition rate, but also improved the indexes of spleen and thymus and the levels of IgG and IgM. Similarly, the CTX in combination with different doses of betacyanin had a larger number of white blood cell (WBC), platelet (PLT) and bone marrow karyocytes than CTX group. Furthermore, betacyanin application also alleviated the CTX-induced increase of alanine aminotransferase (ALT) and blood urea nitrogen (BUN) in S_(180) mice. Conclusion Betacyanins extracted from Portulaca oleracea L. can enhance therapeutic effect of CTX and reduce its side-effect in the S_(180) mice.
