CAJ | 학술논문

目的:探讨免疫抑制剂作为DNA疫苗佐剂的免疫效果及预防自主免疫疾病的效果.方法:将三种免疫抑制剂环孢素(C&A)、他克莫斯(FK506)和霉酚酸酯(MMF)作为多发性硬化症(MS)的DNA疫苗佐剂,共同肌肉注射免疫小鼠.取小鼠脾脏抗体染色后,用流式细胞仪检测调节性T细胞(Treg)的比例及T细胞增殖反应,检测树突细胞(DCs)成熟状态及分泌白介素10(IL-10)的变化,并在诱导EAE动物模型后,检测临床打分的变化.结果:免疫FK506作为MS的DNA疫苗佐剂组显著提高了Treg细胞的比例,T细胞增殖反应显著受到了抑制,DCs细胞成熟受到抑制且IL-10的表达显著升高,能够显著降低小鼠实验性变态反应性脑脊髓炎(EAE)的临床打分.结论:FKS06能够作为DNA疫苗的佐剂,诱导Treg细胞的产生,使机体产生免疫耐受,并能够在一定程度上预防自主免疫疾病的发生,为自身免疫性疾病的预防和治疗提供新的方法和思路.
목적:탐토면역억제제작위DNA역묘좌제적면역효과급예방자주면역질병적효과.방법:장삼충면역억제제배포소(C&A)、타극막사(FK506)화매분산지(MMF)작위다발성경화증(MS)적DNA역묘좌제,공동기육주사면역소서.취소서비장항체염색후,용류식세포의검측조절성T세포(Treg)적비례급T세포증식반응,검측수돌세포(DCs)성숙상태급분비백개소10(IL-10)적변화,병재유도EAE동물모형후,검측림상타분적변화.결과:면역FK506작위MS적DNA역묘좌제조현저제고료Treg세포적비례,T세포증식반응현저수도료억제,DCs세포성숙수도억제차IL-10적표체현저승고,능구현저강저소서실험성변태반응성뇌척수염(EAE)적림상타분.결론:FKS06능구작위DNA역묘적좌제,유도Treg세포적산생,사궤체산생면역내수,병능구재일정정도상예방자주면역질병적발생,위자신면역성질병적예방화치료제공신적방법화사로.
Objective:To check the effect of immunosuppressants as adjuvants for DNA vaccine to prevent autoimmune disease.Methotis:Three immunosuppressants,Cyclosporin A(CsA),Tacrolimus(FK506)and Mycophenolate Mofetil(MMF)Were combined with DNA vaccine of Multiple Sclerosis(MS)and injected intramuscuarly.The splenocytes of immunized mice were checked for regulatory T cells(Treg),T cell proliferation and maturation of dendritic cells(Dcs)as well as release of intereleukin 10(IL-10).The immunized mice were induced as EAE animal model to check the preventive effect of FK506 as adjuvant.Results:More Treg cells Were induced in the mice immunized with FK506 and DNA vaccine and also T cell proliferation Was suppressed.DCs maturation Was blocked and high level of IL-10 in DCs Was induced in mice treated with FK506 and DNA vaccine.The clinic scores of EAE were significantly decreased in mice treated with FK506 and DNA vaccine compared with other control groups.Conclusion:FK506 as adjuvant of DNA vaccine can stimulate Treg cells and immune tolerance,then prevent from the autoimmune disease.