CAJ | 학술논문

建立同时测定反应香精中2-胺基-3,4,8-三甲基-3H-咪唑并[4,5-F]喹喔啉(4,8-DiMeIQx)和2-胺基-1-甲基-6-苯基咪唑[4,5-b]嘧啶(PhIP)的高效液相色谱(HPLC)分析方法.用强酸水解反应香精,在碱性环境下用二氯甲烷萃取分析物,弱酸净化.以乙腈和pH3.44的醋酸-醋酸胺缓冲溶液梯度洗脱,264nm(测定4,8-DiMeIQx)和312 nm(测定PhIP)双波长扫描,进行高效液相色谱检测.4,8-DiMeIQx和PhlP在反应香精中的检出限均为0.01mg/L;加标回收率分别为87.25%和102%.用该法对国内不同公司的不同产品进行检验适用性良好.
건립동시측정반응향정중2-알기-3,4,8-삼갑기-3H-미서병[4,5-F]규악람(4,8-DiMeIQx)화2-알기-1-갑기-6-분기미서[4,5-b]밀정(PhIP)적고효액상색보(HPLC)분석방법.용강산수해반응향정,재감성배경하용이록갑완췌취분석물,약산정화.이을정화pH3.44적작산-작산알완충용액제도세탈,264nm(측정4,8-DiMeIQx)화312 nm(측정PhIP)쌍파장소묘,진행고효액상색보검측.4,8-DiMeIQx화PhlP재반응향정중적검출한균위0.01mg/L;가표회수솔분별위87.25%화102%.용해법대국내불동공사적불동산품진행검험괄용성량호.
A high-performance liquid chromatography(HPLC)analytical method for determination of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine(PhIP)and 2-amino-3,4,8-trimethyl-3H-imidazo[4,5-F]quino xaline(4,8-DiMeIQx)in process flavor was estalliohed.The process flavor samples were hydrolyzed by a strong acid.The sample was extracted from the solution after tFeatment by dichloromethane in alkaline environment,and then purified by a weak acid.Acetonitrile and PH3.44 acetic acid-ammonium acetate buffer solution were used as the mobile phase,and 264 am(determining 4.8-DiMeIQx)and 312nm(determining PhIP)were the detective wavelengths.The detective limitation values for the two heteroeyclic amines(HCAs)in process flavor were both 0.01 mg/L.and the recovery wes 87.25%for 4,8-DiMeIQx and 102%for PhIP.This technique was usable for the determination of process flavor products supplied from different companies.