中国抗生素杂志
中國抗生素雜誌
중국항생소잡지
CHINESE JOURNAL OF ANTIBIOTICS
2010年
3期
228-233
,共6页
华德兴%彭青%曾香连%姚芬%钱元恕
華德興%彭青%曾香連%姚芬%錢元恕
화덕흥%팽청%증향련%요분%전원서
耐甲氧西林金黄色葡萄球菌%茶%绿茶%抗菌
耐甲氧西林金黃色葡萄毬菌%茶%綠茶%抗菌
내갑양서림금황색포도구균%다%록다%항균
Methicillin resistant S.aureus%tea%Green tea%Antibacterial
目的 研究绿茶及提取物对耐甲氧西林金黄色葡萄球菌(MRSA)的抗菌作用并分析其主要抗菌成分.方法 采用琼脂稀释法测定各种茶叶的水浸出物及茶叶的主要成分对MRSA的MIC.纸片扩散法观察绿茶浸出物和茶叶主要成份与抗生素合用对MRSA的协同抗菌情况.结果 ①绿茶浸出物对MRSA的MIC范围为0.125-0.5%,青茶浸出物为0.125-0.5%,白茶浸出物为0.0625-0.25%,红茶浸出物为0.5%,黑茶浸出物为0.25-0.5%.②茶多酚对MRSA的MIC范围为128-256μg/mL,茶色素为256-512μg/mL,茶多糖为512-1024μg/mL,茶皂素和咖啡因则均大于1024μg/mL.③青霉素、苯唑西林、氨苄西林、头孢他啶、头孢替唑、米诺环素、四环素与绿茶浸出物合用的抑菌圈明显大于单用抗生素的抑菌圈,红霉素和氯霉素单用/合用的抑菌圈相同,环丙沙星加入绿茶浸出物后抑菌圈反而减小.④茶多糖、茶皂素、茶色素、咖啡因与苯唑西林单用/合用的抑菌圈均相同,茶多酚与苯唑西林合用的抑菌圈明显大于单用苯唑西林的抑菌圈,表儿茶素没食子酸酯(ECg)/表没食子儿茶素没食子酸酯(EGCg)与苯唑西林合用的抑菌圈明显大于各自单用的抑菌圈.结论 各种茶叶对MRSA均有一定的抗菌作用,其中绿茶和白茶的抗菌作用最强.绿茶抗MRSA的主要成分为茶多酚,并且茶多酚对β-内酰胺类抗生素和四环素抗MRSA有增效作用,但对其它类抗生素呈无关或拮抗作用.茶多酚中的2个单体ECg和EGCg能增强苯唑西林的抗MRSA作用.
目的 研究綠茶及提取物對耐甲氧西林金黃色葡萄毬菌(MRSA)的抗菌作用併分析其主要抗菌成分.方法 採用瓊脂稀釋法測定各種茶葉的水浸齣物及茶葉的主要成分對MRSA的MIC.紙片擴散法觀察綠茶浸齣物和茶葉主要成份與抗生素閤用對MRSA的協同抗菌情況.結果 ①綠茶浸齣物對MRSA的MIC範圍為0.125-0.5%,青茶浸齣物為0.125-0.5%,白茶浸齣物為0.0625-0.25%,紅茶浸齣物為0.5%,黑茶浸齣物為0.25-0.5%.②茶多酚對MRSA的MIC範圍為128-256μg/mL,茶色素為256-512μg/mL,茶多糖為512-1024μg/mL,茶皂素和咖啡因則均大于1024μg/mL.③青黴素、苯唑西林、氨芐西林、頭孢他啶、頭孢替唑、米諾環素、四環素與綠茶浸齣物閤用的抑菌圈明顯大于單用抗生素的抑菌圈,紅黴素和氯黴素單用/閤用的抑菌圈相同,環丙沙星加入綠茶浸齣物後抑菌圈反而減小.④茶多糖、茶皂素、茶色素、咖啡因與苯唑西林單用/閤用的抑菌圈均相同,茶多酚與苯唑西林閤用的抑菌圈明顯大于單用苯唑西林的抑菌圈,錶兒茶素沒食子痠酯(ECg)/錶沒食子兒茶素沒食子痠酯(EGCg)與苯唑西林閤用的抑菌圈明顯大于各自單用的抑菌圈.結論 各種茶葉對MRSA均有一定的抗菌作用,其中綠茶和白茶的抗菌作用最彊.綠茶抗MRSA的主要成分為茶多酚,併且茶多酚對β-內酰胺類抗生素和四環素抗MRSA有增效作用,但對其它類抗生素呈無關或拮抗作用.茶多酚中的2箇單體ECg和EGCg能增彊苯唑西林的抗MRSA作用.
목적 연구록다급제취물대내갑양서림금황색포도구균(MRSA)적항균작용병분석기주요항균성분.방법 채용경지희석법측정각충다협적수침출물급다협적주요성분대MRSA적MIC.지편확산법관찰록다침출물화다협주요성빈여항생소합용대MRSA적협동항균정황.결과 ①록다침출물대MRSA적MIC범위위0.125-0.5%,청다침출물위0.125-0.5%,백다침출물위0.0625-0.25%,홍다침출물위0.5%,흑다침출물위0.25-0.5%.②다다분대MRSA적MIC범위위128-256μg/mL,다색소위256-512μg/mL,다다당위512-1024μg/mL,다조소화가배인칙균대우1024μg/mL.③청매소、분서서림、안변서림、두포타정、두포체서、미낙배소、사배소여록다침출물합용적억균권명현대우단용항생소적억균권,홍매소화록매소단용/합용적억균권상동,배병사성가입록다침출물후억균권반이감소.④다다당、다조소、다색소、가배인여분서서림단용/합용적억균권균상동,다다분여분서서림합용적억균권명현대우단용분서서림적억균권,표인다소몰식자산지(ECg)/표몰식자인다소몰식자산지(EGCg)여분서서림합용적억균권명현대우각자단용적억균권.결론 각충다협대MRSA균유일정적항균작용,기중록다화백다적항균작용최강.록다항MRSA적주요성분위다다분,병차다다분대β-내선알류항생소화사배소항MRSA유증효작용,단대기타류항생소정무관혹길항작용.다다분중적2개단체ECg화EGCg능증강분서서림적항MRSA작용.
Objective To study on antibacterial activity of green tea and its activity against MRSA, and to analyze its main components against MRSA. Methods The MICs of several kinds of tea and the main components of tea against MRSA were performed by agar dilution method. Synergetic antibacterial test of green tea leachate or tea main components with antibiotics against MRSA were carried out by Kirby-Bauer. Results ① The MIC range of green tea leachate was from 0.125% to 0.5%, oolong tea was from 0.125% to 0.5%, white tea was from 0.0625% to 0.25%, dark tea was from 0.25% to 0.5% and black tea was 0.5%. ② The MIC values of tea polyphenol were 128-256μg/mL, values of tea Pigment were 256- 512μg/mL, values of tea polysaccharide were 512-1024μg/mL, and values of tea saponin and caffein were exceeded 1,024ttg/mL. ③ Inhibition zone of penicillin, oxacillin, ampicillin, ceftazedime, ceftezole, minocycline and tetracycline in combination with green tea leachate against MRSA were obviously larger than the single antibiotics. Inhibition zone of erythrocin and chloromycetin against MRSA were not increased when added green tea leachate. While inhibition zone of ciprofloxacin against MRSA were decreased after adding green tea leachate. Inhibition zone of tea polyphenol in combination with Oxacillin against MRSA were obviously larger than the single drug, and ECg/EGCg in combination with Oxacillin against MRSA were also obviously larger than the single drug. Conclusion All kinds of tea show anti-MRSA activity, and green tea and white tea show strongest anti-MRSA activity. The main component against MRSA in green tea is tea polyphenol, and tea polyphenol could enhance the anti-MRSA activity of β-lactam antibiotics and Tetracycline. Two monomers of tea polyphenol, ECg and EGCg, show the ability to enhance the anti-MRSA activity of oxacillin .
