中华临床感染病杂志
中華臨床感染病雜誌
중화림상감염병잡지
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
2011年
2期
87-90
,共4页
陈宝焕%时艳梅%严倩%林爽
陳寶煥%時豔梅%嚴倩%林爽
진보환%시염매%엄천%림상
乳头状瘤病毒感染%宫颈病变%生物状态%L1蛋白
乳頭狀瘤病毒感染%宮頸病變%生物狀態%L1蛋白
유두상류병독감염%궁경병변%생물상태%L1단백
Papillomavirus infections%Cervical lesion%Biological status%L1 protein
目的 探讨人乳头状瘤病毒(HPV)16的存在状态及晚期蛋白L1表达与宫颈病变程度的关系.方法 收集61例宫颈病变患者为研究对象,按病理学诊断结果分为慢性宫颈炎组(27例),CIN Ⅰ、CINⅡ组(10例),原位癌组(8例),早期浸润癌组(7例)和中晚期癌组(9例).采用PCR方法检测HPV分型及生物状态,采用免疫印迹法(Western blot)测定晚期蛋白L1表达.组间比较采用Kruskal-Wallis检验,HPV16生物状态、L1蛋白表达水平与病变程度的关系采用Pearson相关分析.结果 61例宫颈病变患者检出HPV16型53例,占86.9%,其中25例慢性宫颈炎患者均为游离型;CIN Ⅰ、CINⅡ组6例游离型,2例整合型;原位癌组1例游离型,3例混合型和3例整合型;早期浸润癌组2例混合型,4例整合型;中晚期癌组1例混合型,6例整合型.HPV16整合型与宫颈病变程度呈正相关(r=0.705,P＜0.01).随着宫颈病变损伤程度加重,L1蛋白表达逐渐减弱,与宫颈病变程度呈负相关(r=-0.755,P＜0.01).结论 HPV16整合型及L1蛋白表达下降预示宫颈病变程度加重.
目的 探討人乳頭狀瘤病毒(HPV)16的存在狀態及晚期蛋白L1錶達與宮頸病變程度的關繫.方法 收集61例宮頸病變患者為研究對象,按病理學診斷結果分為慢性宮頸炎組(27例),CIN Ⅰ、CINⅡ組(10例),原位癌組(8例),早期浸潤癌組(7例)和中晚期癌組(9例).採用PCR方法檢測HPV分型及生物狀態,採用免疫印跡法(Western blot)測定晚期蛋白L1錶達.組間比較採用Kruskal-Wallis檢驗,HPV16生物狀態、L1蛋白錶達水平與病變程度的關繫採用Pearson相關分析.結果 61例宮頸病變患者檢齣HPV16型53例,佔86.9%,其中25例慢性宮頸炎患者均為遊離型;CIN Ⅰ、CINⅡ組6例遊離型,2例整閤型;原位癌組1例遊離型,3例混閤型和3例整閤型;早期浸潤癌組2例混閤型,4例整閤型;中晚期癌組1例混閤型,6例整閤型.HPV16整閤型與宮頸病變程度呈正相關(r=0.705,P＜0.01).隨著宮頸病變損傷程度加重,L1蛋白錶達逐漸減弱,與宮頸病變程度呈負相關(r=-0.755,P＜0.01).結論 HPV16整閤型及L1蛋白錶達下降預示宮頸病變程度加重.
목적 탐토인유두상류병독(HPV)16적존재상태급만기단백L1표체여궁경병변정도적관계.방법 수집61례궁경병변환자위연구대상,안병이학진단결과분위만성궁경염조(27례),CIN Ⅰ、CINⅡ조(10례),원위암조(8례),조기침윤암조(7례)화중만기암조(9례).채용PCR방법검측HPV분형급생물상태,채용면역인적법(Western blot)측정만기단백L1표체.조간비교채용Kruskal-Wallis검험,HPV16생물상태、L1단백표체수평여병변정도적관계채용Pearson상관분석.결과 61례궁경병변환자검출HPV16형53례,점86.9%,기중25례만성궁경염환자균위유리형;CIN Ⅰ、CINⅡ조6례유리형,2례정합형;원위암조1례유리형,3례혼합형화3례정합형;조기침윤암조2례혼합형,4례정합형;중만기암조1례혼합형,6례정합형.HPV16정합형여궁경병변정도정정상관(r=0.705,P＜0.01).수착궁경병변손상정도가중,L1단백표체축점감약,여궁경병변정도정부상관(r=-0.755,P＜0.01).결론 HPV16정합형급L1단백표체하강예시궁경병변정도가중.
Objective To evaluate the relationship of biological status of HPV 16 and expression of L1 protein with the degree of cervical tumorigenesis.Methods Sixty-one patients with cervical lessions were enrolled and divided into five groups according to pathology of cervical lesions,including chronic cervicitis(n = 27),cervical intraepithelial neoplasia(CIN) Ⅰ-Ⅱ(n = 10),carcinoma in situ(n = 8),early-stage(n =7)and mid/late-stage(n = 9)of cervical carcinoma.HPV type and its biological status were detected by PCR amplification,and L1 protein in HPV 16 positive tissues was detected by Western blot.Kruskal-Wallis was used to compare between the groups,and Pearson correlation analysis was used to evaluate the relationship of HPV biological status and L1 protain expression with cervical lesions.Results Fifty-three out of 61 patients with cervical lesions were detected with HPV positive(86.9%).All 25 patients with chronic cervicitis were in HPV free mode; in CIN Ⅰ-Ⅱ group,6 patients were in free and 2 in integral mode; in carcinoma in situ group,1 patient in free,3 in mixed and 3 in integral mode; in the early-stage cervical cancer group,2 patients in mixed and 4 in integral mode; in mid/late-stage cancer group,1 patient in mixed and 6 in integral mode.A strong positive correlation was found between the HPV in integral mode and the severity of cervical lesions(r = 0.705,P ＜0.01).The expression of L1 protein was negatively correlated with the aggravation of cervical lesions in HPV 16-positive patients(r = -0.755,P ＜0.01).Conclusion The integral mode of HPV16 and low expression of L1 protein may have predictive value for the severity of cervical lesions.
