CAJ | 학술논문

目的评价淋球菌opa分型及Ng-MAST对淋球菌分离株进行基因分型的能力,分析性伴分离淋球菌基因型别之间的关系.方法本研究中24株淋球菌野生株于2006年2月至2007年8月从华中科技大学同济医学院附属协和医院皮肤科门诊分离获得,经Thayer Martin(T-M)培养基选择分离培养后通过革兰染色和氧化酶试验等方法确证.PCR扩增其opa基因以Hpa Ⅱ酶切及聚丙烯酰胺凝胶电泳银染显色分析,并用NG-MAST方法从分子水平上进行基因分型.结果 24株野生株淋球菌中,Ng-MAST区分出10种ST型,而使用opa分型则呈现12种OT型;新发现的ST(217-86%同源178)是国内特有的流行株.结论淋病患者一性伴组除了45/46之外,每对菌株的ST型及OT型均相同,表明患者与性伴相互接触传染.淋球菌opa分型能力高于Ng-MAST,ST型别可进一步的分成opa*types(OT)亚型.
목적 평개림구균opa분형급Ng-MAST대림구균분리주진행기인분형적능력,분석성반분리림구균기인형별지간적관계.방법 본연구중24주림구균야생주우2006년2월지2007년8월종화중과기대학동제의학원부속협화의원피부과문진분리획득,경Thayer Martin(T-M)배양기선택분리배양후통과혁란염색화양화매시험등방법학증.PCR확증기opa기인이Hpa Ⅱ매절급취병희선알응효전영은염현색분석,병용NG-MAST방법종분자수평상진행기인분형.결과 24주야생주림구균중,Ng-MAST구분출10충ST형,이사용opa분형칙정현12충OT형;신발현적ST(217-86%동원178)시국내특유적류행주.결론 임병환자일성반조제료45/46지외,매대균주적ST형급OT형균상동,표명환자여성반상호접촉전염.림구균opa분형능력고우Ng-MAST,ST형별가진일보적분성opa*types(OT)아형.
Objective To compare the potentiality of opa typing versus Neisseria gonorrhoeae multi-antigen sequence typing (Ng-MAST) in discrimination of N. gonorrhoeae isolates, and to investigate the consistency of genotypes of N. gonorrhoeae between patients and their sex partners. Methods N. gonor-rhoeae was isolated from patients and their sex partners who attended sexually transmitted disease clinics of the Affiliated Union Hospital of Tongji Medical College, Huazhong University of Science and Technology from February 2006 to August 2007. All isolates were cultured on Thayer-Martin agar and GC-based choco-late agar, and identified by Gram stain and oxidase reaction. Totally, twenty-four strains of N. gonorrhoeae were isolated form 12 pairs of male-female sex partners. Genomic DNA was isolated from the strains, and subjected to PCR amplification of opa gene as well as pot and tbpB genes, opa typing and Ng-MAST typing were utilized to differentiate the 24 strains respectively. Results The 24 isolates were classified into 10 sequence types (ST) by Ng-MAST typing, and 12 opa types (OT) by opa typing. A novel genotype identi-fied by Ng-MAST (217-86% homologisation 178), was unique in China. Conclusions Both ST and OT of N. gonorrhoeae strains are identical between patients and their corresponding partners except for one pair of partners, 45/46, suggesting that gonorrhea is transmitted to each other by contiguity between sex partners. Opa typing is likely to be more potential than Ng-MAST in genotyping of N.gonorrhoeae strains.