CAJ | 학술논문

本文对32例病理确诊的神经母细胞瘤进行回顾性研究,对所有病理标本重新进行组织学分型,同时进行NSE及s-100蛋白免疫组化染色,并利用全自动图象分析仪对其中28例进行了瘤细胞DNA定量测定及倍体分析.结果表明:NSE及s-100蛋白免疫组化染色可反映神经母细胞瘤分化程度,s-100蛋白阳性细胞的出现是该肿瘤分化成熟及预后良好的表现,DNA异倍体是预后良好的可靠标志.
본문대32례병리학진적신경모세포류진행회고성연구,대소유병리표본중신진행조직학분형,동시진행NSE급s-100단백면역조화염색,병이용전자동도상분석의대기중28례진행료류세포DNA정량측정급배체분석.결과표명:NSE급s-100단백면역조화염색가반영신경모세포류분화정도,s-100단백양성세포적출현시해종류분화성숙급예후량호적표현,DNA이배체시예후량호적가고표지.
Thirty-two children with neuroblastoma (Nb) confirmed by pathological examination were studied retrospectively with Avidin-Biotin's Peroxidase Complex (ABC) method and IBAS-2000 Automatic Image Analysis System (Germany). All the specimens were prepared with neurospecific enolase (NSE) stain and S-100 protein im-munohistochemistry stain. Twenty-eight of them were analysed for DNA content with image analysis system (IAT). According to Joshi's criteria, the specimens were divided into undifferentiated, poorly differentiated and differentated types. Both NSE and S-100 protein stain were weakly postive in undif ferentiated Nb but strongly positive in differentiated Nb. Among the 28 cases of Nb, diploid in 16 (57. 1%), and aneuploid in 12 (42.8%) with a DNA index from 0. 98 to 2. 14 at random. This study suggest that: 1. NES and S-100 protein immunohis-tochemical staining might be useful in evaluating the degree of Nb cell maturation. The presence of S-100 protein positive cells indicates a better prognosis; 2. DNA ploidy analysed by image analysis system might accurately reflect the biological behavior of Nb, and DNA aneuploidy might be an unfavorable outcome.