CAJ | 학술논문

目的探讨大黄素(emodin)诱导细胞凋亡对不同类型急性胰腺炎(AP)腺泡细胞死亡方式影响及其可能机制.方法制作大鼠急性水肿型胰腺炎(AEP)与急性坏死型胰腺炎(ANP)模型.使用大黄素进行治疗.采用原位TUNEL及RT-PCR等技术,观察胰腺组织内细胞凋亡、NF-κBmRNA、Bax mRNA及TNF-α mRNA等变化情况.结果各组大鼠胰腺细胞凋亡指数与胰腺组织病理学评分呈负相关;大黄素治疗可诱导各型AP模型腺泡细胞凋亡,胰腺病理学改变及组织学评分改善,胰腺组织内NF-κB mRNA及Bax mRNA表达增加.结论诱导不同类型AP胰腺细胞凋亡可明显减轻AP病情.大黄素为治疗AP的有效药物,可能通过影响NF-κB活化及Bax的表达来诱导AP时腺泡细胞的凋亡,减轻胰腺炎的严重程度.
목적 탐토대황소(emodin)유도세포조망대불동류형급성이선염(AP)선포세포사망방식영향급기가능궤제.방법 제작대서급성수종형이선염(AEP)여급성배사형이선염(ANP)모형.사용대황소진행치료.채용원위TUNEL급RT-PCR등기술,관찰이선조직내세포조망、NF-κBmRNA、Bax mRNA급TNF-α mRNA등변화정황.결과 각조대서이선세포조망지수여이선조직병이학평분정부상관;대황소치료가유도각형AP모형선포세포조망,이선병이학개변급조직학평분개선,이선조직내NF-κB mRNA급Bax mRNA표체증가.결론 유도불동류형AP이선세포조망가명현감경AP병정.대황소위치료AP적유효약물,가능통과영향NF-κB활화급Bax적표체래유도AP시선포세포적조망,감경이선염적엄중정도.
Objective To investigate the effect of Chinese medicine emodin on death patterns of pancreaticacinar cells in different types of aute pancreatitis and explore its possible mechanism. Methods Rat acute edematous pancreatitis models and acute necrotic pancreatitis models were estab-lished and treated with emodin. Alternations of apoptosis, NF-κB mRNA, Pax mRNA and TNF-α mRNA expression in pancreatic tissue were studied with in situ TUNEL and RT-PCR. Results Nega-tive correlation was found between apoptosis and the severity of pancreatic damage. Following the treatment with emodin, more apoptosis than necrosis was observed in all treaded groups of acute pan-creatitis and the severity of pancreatic histological pathologic damage was dramatically improved. Local expression of NF-κB mRNA and Bax mRNA increased. Conclusion Pancreas aeinar death patterns of apoptosis are induced by emodin. Emodin might regulate the acinar cell death patterns by changing the activation of NF-κB and the expression of bax to lessen the severity.