CAJ | 학술논문

目的检测系统性红斑狼疮(SLE)患者血浆中差异表达的微小RNAs(miRNAs),为寻求一种新的无创性SLE生物标志物奠定基础.方法采用Agilent human miRNA芯片检测并筛选出SLE患者与健康人血浆中表达丰度有显著变化miRNA,并通过实时荧光定量聚合酶链反应(RT-qPCR)对部分差异表达基因进行验证.2组间的比较用独立样本的t检验.结果 MiRNAs芯片检测发现,SLE患者与健康对照间存在明显差异的循环miRNAs共有51个,其中19个上调,32个下调；RT-qPCR对其中4个上调(miR- 126、miR-21、miR-223和miR-451)和3个下调(miR-125a-3p、miR-146a和miR-155)循环miRNAs的验证结果与芯片数据所示具有较好的一致性.结论 SLE患者和健康人体内循环miRNAs的表达谱存在着明显差异,这些差异的循环miRNAs可作为一种潜在的SLE候选生物标志物.
목적 검측계통성홍반랑창(SLE)환자혈장중차이표체적미소RNAs(miRNAs),위심구일충신적무창성SLE생물표지물전정기출.방법 채용Agilent human miRNA심편검측병사선출SLE환자여건강인혈장중표체봉도유현저변화miRNA,병통과실시형광정량취합매련반응(RT-qPCR)대부분차이표체기인진행험증.2조간적비교용독립양본적t검험.결과 MiRNAs심편검측발현,SLE환자여건강대조간존재명현차이적순배miRNAs공유51개,기중19개상조,32개하조；RT-qPCR대기중4개상조(miR- 126、miR-21、miR-223화miR-451)화3개하조(miR-125a-3p、miR-146a화miR-155)순배miRNAs적험증결과여심편수거소시구유교호적일치성.결론 SLE환자화건강인체내순배miRNAs적표체보존재착명현차이,저사차이적순배miRNAs가작위일충잠재적SLE후선생물표지물.
Objective To investigate a panel of differentially expressed circulating microRNAs (miRNAs) as potential biomarkers in patients with systemic lupus erythematosus (SLE).Methods A miRNA array was performed on plasma of 10 healthy controls and 10 SLE patients.To confirm the results of microarray,the selected 7 miRNAs were examined by real-time quantitative PCR (RT-qPCR).Independent sample's t-test was used for statistical analysis between the two groups.Results A total of 51 circulating miRNAs were signi-ficandy differentially expressed between SLE patients and healthy controls (19 up-regulated miRNAs and 32 down-regulated miRNAs).The findings of RT-qPCR on 7 miRNAs (miR-126,miR-21,miR-223 and miR-451 of upregulation and miR-125a-3p,miR-146a and miR-155 of down-regulation) were consistent with the data obtained from the array.Conclusion There is aspecific circulating miRNAs expression profile in SLE,and these aberrantly expressed miRNAs might have great potential to serve as novel,noninvasive biomarkers of SLE.