CAJ | 학술논문

目的探讨儿童变应性鼻炎(allergic rhinitis,AR)严重度与转化生长因子β1(transforming growth factor β1,TGF-β1)基因(TGFB1)启动子区-509C/T多态性的相关性.方法儿童持续性AR患者96例,年龄3～17岁,平均((x)±s)年龄(9.4±3.8)岁;其中轻度51例(53.1%),中-重度45例(46.9%).应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测患儿外周血中TGFB1基因-509C/T多态性的基因型,双抗体夹心ELISA法测定血清TGF-β1水平,ImmunoCAP100E变应原体外检测系统测定血清总IgE、特异性IgE和嗜酸粒细胞阳离子蛋白(eosinophil cationic protein,ECP)水平.使用SPSS11.0统计学软件对实验结果进行分析.结果 TGFB1基因-509C/T多态性在轻度AR组和中-重度AR组中的基闪型分布差异有统计学意义(x2=8.361,P=0.015),TT基因型与CC/CT基因型相比,在中-重度AR组中的频率显著高于轻度AR组(Fisher's精确概率法,P=0.007).该多态性位点的各基凶型之间,患者血清TGF-β1水平的差异无统计学意义(F=0.389,P=0.679),但血清总IgE水平(F=4.210,P=0.018)和ECP水平(H=6.297,P=0.043)的差异均有统计学意义.结论 TGFB1基因-509C/T多态件对儿童持续性AR的严重度可能有一定影响.
목적 탐토인동변응성비염(allergic rhinitis,AR)엄중도여전화생장인자β1(transforming growth factor β1,TGF-β1)기인(TGFB1)계동자구-509C/T다태성적상관성.방법 인동지속성AR환자96례,년령3～17세,평균((x)±s)년령(9.4±3.8)세;기중경도51례(53.1%),중-중도45례(46.9%).응용취합매련반응-한제성편단장도다태성(PCR-RFLP)기술검측환인외주혈중TGFB1기인-509C/T다태성적기인형,쌍항체협심ELISA법측정혈청TGF-β1수평,ImmunoCAP100E변응원체외검측계통측정혈청총IgE、특이성IgE화기산립세포양리자단백(eosinophil cationic protein,ECP)수평.사용SPSS11.0통계학연건대실험결과진행분석.결과 TGFB1기인-509C/T다태성재경도AR조화중-중도AR조중적기섬형분포차이유통계학의의(x2=8.361,P=0.015),TT기인형여CC/CT기인형상비,재중-중도AR조중적빈솔현저고우경도AR조(Fisher's정학개솔법,P=0.007).해다태성위점적각기흉형지간,환자혈청TGF-β1수평적차이무통계학의의(F=0.389,P=0.679),단혈청총IgE수평(F=4.210,P=0.018)화ECP수평(H=6.297,P=0.043)적차이균유통계학의의.결론 TGFB1기인-509C/T다태건대인동지속성AR적엄중도가능유일정영향.
Objective To investigate the association between the promoter polymorphism -509C/T of the transforming growth factor-pi gene (TGFB1) and the disease severity of allergic rhinitis (AR) in childhood. Methods A total of 96 Chinese patients with persistent AR aged 3-17 (9. 4 ±3. 8) years old were enrolled in the study. Among these patients 53. 1% were mild cases (n = 51 ) and 46.9% were moerate-to-severe cases (n = 45). Genotyping was performed on peripheral blood genomic DNA by using PCR-RFLP. Serum levels of TGF-β1 was measured by ELISA, and serum total IgE, specific IgE and eosinophil cationic protein (ECP) levels were determined using an ImmunoCAPlOOE system. Statistical analysis was conducted with SPSS11.0 software. Results Significant differences were found in genotype frequencies for the TGFB1 - 509C/T polymorphism between mild and moderate-to-severe AR patients (X2 = 8. 361 , P =0. 015 ). Children with persistent AR bearing the TT genotype of the - 509C/T polymorphism had significantly increased risk for moderate-to-severe AR (Fisher's exact test, P=0.007) compared to children with the CC/CT genotypes. There was no significant association between the - 509C/Tpolymorphism and serum TGF-β1 levels (F=0. 389,P=0. 679) ; however, serum total IgE (F = 4.210,P = 0.018) and ECP ( H = 6. 297, P = 0. 043) levels were found to be significantly associated with the polymorphism. Conclusion The results suggest that the TGFB1 gene polymorphism - 509C/T may play a potential role in the severity of persistent AR in childhood.