CAJ | 학술논문

目的探讨氧化应激对于脂肪细胞因子脂联素、瘦素、抵抗素和内脏脂肪素表达和分泌的影响.方法体外培养小鼠3T3-L1前脂肪细胞,并诱导其分化成熟.通过在高糖培养基中加入葡萄糖激酶制作氧化应激模型.分为4个试验组,分为四个试验组,A组:观察不同浓度的葡萄糖激酶对七述脂肪细胞因子的影响;B组:不同浓度的抗氧化应激物N乙酰半胱氨酸(NAC)拮抗氧化应激后,对脂肪细胞因子的作用;C组:NAC作用不同时间对抗氧化应激作用的影响;D组:观察氧化应激伴或不伴拮抗效应对脂肪细胞因子的影响.结果 (1)脂联素、瘦素、抵抗素和内脏脂肪素的表达随着葡萄糖激酶浓度的增加呈现浓度依赖性抑制,其中脂联素和瘦素的这种效应更为明显[葡萄糖激酶浓度25 U/L时,脂联素水平达(6.94±0.07)ng/L,瘦素水平达(0.64±0.11)ng/L,与空白对照组相比,均P<0.01];(2)随着NAC作用持续时间的延长,脂联素、瘦素、抵抗素和内脏脂肪素表达增加[NAC作用16 h,脂联素水平达(19.22±0.27)ng/L,瘦素水平达(2.95±0.22)ng/L,与空白对照组相比,均P<0.01];(3)在同一葡萄糖激酶浓度下(25 U/L),随着NAC浓度的增加,脂联素、瘦素、抵抗素和内脏脂肪素表达旱现浓度依赖性增加(与空白对照组相比,NAC 25 mmol/L组脂联素、瘦素、抵抗素和内脏脂肪素t值分别为6.88、6.96、4.52、3.15,均P<0.05);(4)与空白对照组相比,NAC作用组的脂联素、瘦素、抵抗素和内脏脂肪素表达明显增加,葡萄糖激酶作用组脂联素、瘦素、抵抗素和内脏脂肪素表达明显减少.结论氧化应激对于脂肪细胞因子的表达有明显影响,对抗氧化应激可改善这种作用.此作用可能是氧化应激参与胰岛索抵抗和代谢功能紊乱的机制之一.
목적 탐토양화응격대우지방세포인자지련소、수소、저항소화내장지방소표체화분비적영향.방법 체외배양소서3T3-L1전지방세포,병유도기분화성숙.통과재고당배양기중가입포도당격매제작양화응격모형.분위4개시험조,분위사개시험조,A조:관찰불동농도적포도당격매대칠술지방세포인자적영향;B조:불동농도적항양화응격물N을선반광안산(NAC)길항양화응격후,대지방세포인자적작용;C조:NAC작용불동시간대항양화응격작용적영향;D조:관찰양화응격반혹불반길항효응대지방세포인자적영향.결과 (1)지련소、수소、저항소화내장지방소적표체수착포도당격매농도적증가정현농도의뢰성억제,기중지련소화수소적저충효응경위명현[포도당격매농도25 U/L시,지련소수평체(6.94±0.07)ng/L,수소수평체(0.64±0.11)ng/L,여공백대조조상비,균P<0.01];(2)수착NAC작용지속시간적연장,지련소、수소、저항소화내장지방소표체증가[NAC작용16 h,지련소수평체(19.22±0.27)ng/L,수소수평체(2.95±0.22)ng/L,여공백대조조상비,균P<0.01];(3)재동일포도당격매농도하(25 U/L),수착NAC농도적증가,지련소、수소、저항소화내장지방소표체한현농도의뢰성증가(여공백대조조상비,NAC 25 mmol/L조지련소、수소、저항소화내장지방소t치분별위6.88、6.96、4.52、3.15,균P<0.05);(4)여공백대조조상비,NAC작용조적지련소、수소、저항소화내장지방소표체명현증가,포도당격매작용조지련소、수소、저항소화내장지방소표체명현감소.결론 양화응격대우지방세포인자적표체유명현영향,대항양화응격가개선저충작용.차작용가능시양화응격삼여이도색저항화대사공능문란적궤제지일.
Objective To investigate the influence of oxidative stress on expression and secretion of adiponectin, leptin, resisitin and visfatin in adipocytes. Methods In vitro culture 3T3-L1 preadipocyte, induce the cell differentiation and maturity. Adding glucokinase into high-glucose culture media to make oxidative modeL The cultured cells were divided into four groups for experiment, Group A: the expression of adipanectin, leptin, resistin and visfatin were examined in different concentration of glucokinase; Group B: the expression of above mentioned 4 adipocytokines were examined after against oxidative stress in different concentration of N - acetyl eysteine(NAG) ; Group C : the effect of different time period of NAC against the oxidative stress; Group D: oxidative stress with or without antagonistic effect on the above 4 adipocytokines.Results (1) With the increase of glucokinase concentration, concentration-dependent inhibition of expression were observed with adiponectin, leptin, visfatin and resistin. Among these, adiponectin and ghcokinase concentration of 25 U/L, all P <0.01 compared with control). (2) With the extension of NAC incubation duration, the expression of adiponectin, leptin, resistin and visfatin were increased (adiponectin with control). (3) With the same concentration of glucose oxidase (25 U/L), concentration-dependent increase of adipokine expression were observed with the increase of NAC concentration(adiponectin t = 6.88, leptin t = 6.96, resistin t = 4.52, visfatin t = 3.15 with NAC concentration of 25 mmol/L, all P < 0.05) . (4) Compared with the blank control, the expression of adiponectin, leptin, visfatin and resistin in NAC group were increased significantly, the expression of the adipokines were decreased significantly in glucokinase group. Conclusions Oxidative stress has significant influence on adiponectin, leptin, resistin and visfatin expression in adipocytes. Anti-oxidative can improve this effect. The influence of oxidative stress on adipokines maybe one of the mechanisms of insulin resistance and metabolic dysfunction pathogenesis.