CAJ | 학술논문

目的以支气管肺泡灌洗液(BALF)中NO水平和白细胞为指标,观察豚鼠定量吸烟后肺、气道的迟发性反应;以及很低剂量的哌替啶对这些反应的抑制作用.方法经吸烟装置,豚鼠自主吸入60mL香烟烟雾(75%浓度)/次,共6次,每次间隔20min.2h后检测BALF中白细胞数及其分类,以及BALF中NO2ˉ/ NO3ˉ的浓度,以反映气道内NO水平.同时在药物组中观察很低剂量哌替啶对上述反应的抑制作用.结果豚鼠定量吸烟2h后,BALF中细胞数、中性粒细胞百分比和NO2ˉ/ NO3ˉ水平显著增高;哌替啶0.1mg·kg-1能明显抑制上述反应.结论多次吸烟2h后,豚鼠肺、气道出现神经源性的迟发性炎症反应,很低剂量的哌替啶有效地抑制豚鼠吸烟产生的迟发性炎症反应.
목적이지기관폐포관세액(BALF)중NO수평화백세포위지표,관찰돈서정량흡연후폐、기도적지발성반응;이급흔저제량적고체정대저사반응적억제작용.방법경흡연장치,돈서자주흡입60mL향연연무(75%농도)/차,공6차,매차간격20min.2h후검측BALF중백세포수급기분류,이급BALF중NO2ˉ/ NO3ˉ적농도,이반영기도내NO수평.동시재약물조중관찰흔저제량고체정대상술반응적억제작용.결과돈서정량흡연2h후,BALF중세포수、중성립세포백분비화NO2ˉ/ NO3ˉ수평현저증고;고체정0.1mg·kg-1능명현억제상술반응.결론다차흡연2h후,돈서폐、기도출현신경원성적지발성염증반응,흔저제량적고체정유효지억제돈서흡연산생적지발성염증반응.
OBJECTIVE To investigate the delayed responses after acute smoking, we observed leukocyte and nitric oxide (NO) in BAL fluid in guinea pigs; and to further investigate the inhibitory effect of low-dose pethidine against the delayed response.METHOD Cell counts were determined in BAL fluid after inhalation six volumes of cigarette smoke (60mL, 75% concentration) successively via the smoking device; and the concentration of NO2ˉ/ NO3ˉwas also measured to reflect the level of NO with biochemical method. The inhibitory effect against the above responses of pethidine was investigated in pethidine pre-treated group.RESULTS The total cell numbers, the percentage of neutrophils and the concentration of NO2ˉ/ NO3ˉ in BAL fluid were increased significantly after smoke exposure. Pethidine 0.1mg*kg-1 largely inhibited these responses.CONCLUSION A delayed neurogenic inflammation was observed in airways and parenchymal two hours after smoke exposure; and low-dose of pethidine significantly inhibit this delayed lung injury.