CAJ | 학술논문

文吕鱼的进化地位十分重要,对其染色体的研究在进化和比较基因组学方面有重要意义.然而文昌鱼的染色体制备困难,使研究受到了限制.本文介绍了一种改良的义昌鱼胚胎细胞染色体标本制备方法,以及用文昌鱼成体再生细胞制备染色体,首次获得了文昌鱼体细胞中期染色体标本,并观察了厦门2种文昌鱼的染色体,其中白氏文吕鱼(Branchiostoma belcheri)二倍体2n=40,日本文昌鱼(B.japonicum)二倍体2n=36.再次从细胞分类学角度证实白氏文昌鱼和日本文昌鱼作为两个独立物种的分类地位.
문려어적진화지위십분중요,대기염색체적연구재진화화비교기인조학방면유중요의의.연이문창어적염색체제비곤난,사연구수도료한제.본문개소료일충개량적의창어배태세포염색체표본제비방법,이급용문창어성체재생세포제비염색체,수차획득료문창어체세포중기염색체표본,병관찰료하문2충문창어적염색체,기중백씨문려어(Branchiostoma belcheri)이배체2n=40,일본문창어(B.japonicum)이배체2n=36.재차종세포분류학각도증실백씨문창어화일본문창어작위량개독립물충적분류지위.
Amphioxus has an important evolutionary position as a result of their phylogenetic position relative to vertebrates. Understanding their chromosomes would provide key points in the study of evolutionary biology and comparative genomics. The difficulty in preparing amphioxus chromosomes currently provides a significant hurdle in this research. In the current study, we describe an improved method for metaphase preparation from amphioxus embryos and methodology for preparing metaphase spreads from regenerative somatic cells. Chromosomes of two amphioxus species from Xiamen waters in China are also observed. The diploid chromosome number was found to be 40 in Branchiostoma belcheri, while B.japonicum has 36, confirming the two are distinct species from cytotaxonomic viewpoint.