CAJ | 학술논문

运用RAPD技术对白杨派中7个树种和3个人工杂交种共计50个无性系的亲缘关系进行分析.结果表明:筛选出的26条随机引物共扩增产生222条谱带,其中200条为多态性谱带,多态性带达90.09%,且种间遗传变异大于种内无性系间的遗传变异.聚类分析结果显示,参试的50个材料可分为2大类:第一类由山杨、响叶杨、河北杨组成;第二类由毛白杨、银毛杨、84K杨、银白杨、银灰杨、新疆杨和银新杨组成;3个人工杂交种与白杨亚派树种的亲缘关系较近;人工杂交种银毛杨与84K杨间亲缘关系最近.
운용RAPD기술대백양파중7개수충화3개인공잡교충공계50개무성계적친연관계진행분석.결과표명:사선출적26조수궤인물공확증산생222조보대,기중200조위다태성보대,다태성대체90.09%,차충간유전변이대우충내무성계간적유전변이.취류분석결과현시,삼시적50개재료가분위2대류:제일류유산양、향협양、하북양조성;제이류유모백양、은모양、84K양、은백양、은회양、신강양화은신양조성;3개인공잡교충여백양아파수충적친연관계교근;인공잡교충은모양여84K양간친연관계최근.
The study analyzes the relationship of Sect.Populus by random amplified polymorphic DNA (RAPD) technique.7 species and 3 artificial hybrids were used in this experiment.Every species has 5 different clones except the 3 artificial hybrids.26 primers were selected for RAPD.Among the total of 222 bands,there are 200 bands (90.09%) were detected to be polymorphic.The genetic distances demonstrate that the diversity between all species is larger than that of the clones from every species.The phylogenetic dendrogram shows that all these 10 species were divided into two categories:The first category including P.davidiana Dode,P.adenpoda Maxim.and P.hopeiensis Hu et Chow;and the second category including P.tomentosa Carr.,P.alba×P.tomentosa Carr.,P.alba×P.glandulosa,P.alba L.,P.canescens (Ait.) Smith,P.alba var.pyramidalis Bge.and P.alba×P.alba var.pyramidalis Bge..The three artificial hybrids are closer to Subsect.Albidae Dode.Trepidae Dode,P.alba×P.tomentosa and are the closest in the three artificial hybrids.