基础医学与临床
基礎醫學與臨床
기출의학여림상
BASIC MEDICAL SCIENCES AND CLINICS
2010年
3期
303-305
,共3页
洪岭%滕晓明%李昆明%黄文强%张燕%张博%童晓文
洪嶺%滕曉明%李昆明%黃文彊%張燕%張博%童曉文
홍령%등효명%리곤명%황문강%장연%장박%동효문
IL-6%多囊卵巢综合征%卵泡颗粒细胞%雄激素受体
IL-6%多囊卵巢綜閤徵%卵泡顆粒細胞%雄激素受體
IL-6%다낭란소종합정%란포과립세포%웅격소수체
interleukin 6%PCOS%ovarian granulosa cell%androgen receptor
目的 研究白细胞介素6(IL-6)对卵泡颗粒细胞雄激素受体(AR)表达的影响,探讨IL-6在多囊卵巢综合征(PCOS)发病机制中的作用.方法 在体外培养的猪卵泡颗粒细胞培养液中分别加入10、100和1 000 ng/L的IL-6,采用实时荧光定量PCR和Western blot法观察IL-6对卵巢颗粒细胞AR mRNA和蛋白的表达.结果 IL-6可上调卵巢颗粒细胞AR mRNA和蛋白的表达.结论 IL-6增强雄激素的活性,可能参与了慢性亚临床炎症引发的PCOS.
目的 研究白細胞介素6(IL-6)對卵泡顆粒細胞雄激素受體(AR)錶達的影響,探討IL-6在多囊卵巢綜閤徵(PCOS)髮病機製中的作用.方法 在體外培養的豬卵泡顆粒細胞培養液中分彆加入10、100和1 000 ng/L的IL-6,採用實時熒光定量PCR和Western blot法觀察IL-6對卵巢顆粒細胞AR mRNA和蛋白的錶達.結果 IL-6可上調卵巢顆粒細胞AR mRNA和蛋白的錶達.結論 IL-6增彊雄激素的活性,可能參與瞭慢性亞臨床炎癥引髮的PCOS.
목적 연구백세포개소6(IL-6)대란포과립세포웅격소수체(AR)표체적영향,탐토IL-6재다낭란소종합정(PCOS)발병궤제중적작용.방법 재체외배양적저란포과립세포배양액중분별가입10、100화1 000 ng/L적IL-6,채용실시형광정량PCR화Western blot법관찰IL-6대란소과립세포AR mRNA화단백적표체.결과 IL-6가상조란소과립세포AR mRNA화단백적표체.결론 IL-6증강웅격소적활성,가능삼여료만성아림상염증인발적PCOS.
Objective To study the effect of intedeukin 6 (IL-6) on the expression of androgen receptor (AR) in porcine ovarian granulosa cells(GC) and to explore its role of in the polycystic ovary syndrome(PCOS) pathogenesis.Methods Different concentrations of IL-6 (10,100 and 1 000 ng/L) were treated with GC.Expression of androgen receptor (AR) mRNA and protein in GC were detected by real-time quantitative RT-PCR and Western blot.Results The mRNA and protein expressions of AR in GC were upregulated in groups with 100 and 1 000 ng/L IL-6 at 72 h.Conclusion IL-6 can improve androgen activity.This process may explain the function of chronic subclinical inflammation in PCOS pathogenesis.
