CAJ | 학술논문

[目的]检测大鼠放射性口腔黏膜炎形成过程中病损局部组织内细胞因子,探讨其动态变化规律及意义.[方法]获取接受单次30 Gy x线照射后5 d、8 d、14 d的大鼠口腔黏膜组织,采用抗体芯片技术检测19种细胞因子;荧光定量PCR检测8种炎性相关因子的mRNA水平;ELISA定量检测IL-1α、IFN-γ、TIMP-1.[结果]抗体芯片技术检测到,照射后Frac、IL-4、IL-6、IL-10、TNF-α 5种细胞因子水平下降;IL-1β、LIX、VEGF、β-NGF 4种细胞因子照射前后水平无明显差异;照射后水平上升幅度＜2倍的有CINC-2、GM-CGF、Leptin、MCP-1和MIP-3α 5种细胞因子,上升幅度≥2倍的有CINC-3、INF-γ、IL-1α和TIMP-1 4种细胞因子.Real-time PCR检测到抗凋亡相关基因Bcl-2,以及IL-6、TNF-α、VEGF及IL-1β的mRNA表达水平下降;而促凋亡相关基因Bax、中性粒细胞局部募集相关基因CINC和TLR-9表达上升.IL-1α、IFN-γ、TIMP-1的ELISA检测结果与前两种检测结果一致.[结论]大鼠抗体芯片和荧光定量PER、ELISA检测结果互相佐证了病损组织内的细胞因子在口腔黏膜炎发生、发展、演进中呈现分泌受阻和表达增加的动态变化并发挥调控作用.
[목적]검측대서방사성구강점막염형성과정중병손국부조직내세포인자,탐토기동태변화규률급의의.[방법]획취접수단차30 Gy x선조사후5 d、8 d、14 d적대서구강점막조직,채용항체심편기술검측19충세포인자;형광정량PCR검측8충염성상관인자적mRNA수평;ELISA정량검측IL-1α、IFN-γ、TIMP-1.[결과]항체심편기술검측도,조사후Frac、IL-4、IL-6、IL-10、TNF-α 5충세포인자수평하강;IL-1β、LIX、VEGF、β-NGF 4충세포인자조사전후수평무명현차이;조사후수평상승폭도＜2배적유CINC-2、GM-CGF、Leptin、MCP-1화MIP-3α 5충세포인자,상승폭도≥2배적유CINC-3、INF-γ、IL-1α화TIMP-1 4충세포인자.Real-time PCR검측도항조망상관기인Bcl-2,이급IL-6、TNF-α、VEGF급IL-1β적mRNA표체수평하강;이촉조망상관기인Bax、중성립세포국부모집상관기인CINC화TLR-9표체상승.IL-1α、IFN-γ、TIMP-1적ELISA검측결과여전량충검측결과일치.[결론]대서항체심편화형광정량PER、ELISA검측결과호상좌증료병손조직내적세포인자재구강점막염발생、발전、연진중정현분비수조화표체증가적동태변화병발휘조공작용.
[Objective]This study was designed to explore the dynamic changes of cytokines in the local tissues of radiationinduced oral mucositis in a rat model.[methods]The rat oral mucosal tissues were obtained at 5 d,8 d,and 14 d after irradiation.which were received single X-ray irradiation of 30 Gy locally.RayBio~((R)) Rat Cytokine Array was applied to analyze the 19 cytokines.The mRNA levels of the 8 inflammatory-related cytokines were analyzed by real time-PCR.ELISA was employed to detect IL-1α,IFN-γ,and TIMP-1 protein levels.[Results]Cytokine array detection showed that cytokines such as Frac,IL-4,IL-6,IL-10,and TNF-α were down-regulated after irradiation.IL-1β,LIX,VEGF,and β-NGF were no obviously changed at the same time corse.The elevate range less than 2 fold of the CINC-2,GM-CGF,Leptin,MCP-1,and MIP-3α after irradiation were detected.The increasing range more than 2 fold of CINC-3,INF-γ,IL-1α,and TIMP-1 post irradiation were detected.The mRNA levels of anti-apoptosis,such as Bcl-2,and TNF-α,IL-6,VEGF,and IL-1β were low down,while the levels of promoting apoptosis cytokine such as Bax,and neutrophil recruitment associated cytokines,CINC,and TLR-9 were up-regulated after irradiation.The ELISA results of IL-1α,IFN-γ,and TIMP-1 were identical with the results of the two previom analyses.[Conclusion]The results of the three methods in the study should present evidences to prove each other.Some cytokines in the local lesion of oral mucositis during the initiate,development,and progression stages were blocked,while others were positively up-regulated to involving the control of the pathogensis of oral mucositis.