蚕业科学
蠶業科學
잠업과학
ACTA SERICOLOGICA SINICA
2010年
2期
313-318
,共6页
李卫国%宋尚文%孙明高%苗良%牟志美%冀宪领
李衛國%宋尚文%孫明高%苗良%牟誌美%冀憲領
리위국%송상문%손명고%묘량%모지미%기헌령
桑树%盐分胁迫%渗透调节物质%品种差异
桑樹%鹽分脅迫%滲透調節物質%品種差異
상수%염분협박%삼투조절물질%품충차이
Morus L.%Salt stress%Osmotic regulatory substance%Difference across varieties
以农桑14号、昂绿1号、湖桑32号、鲁插1号4个桑树品种的2年生嫁接苗为材料,研究以不同浓度盐分胁迫不同时间后桑树叶片内渗透调节物质含量的变化.在盐分胁迫下,4个桑树品种叶片中的脯氨酸含量及可溶性糖和可溶性蛋白含量的变化呈现一定规律性,且品种间具有显著差异.4个桑树品种叶片中的脯氨酸含量在各种浓度盐分胁迫初期都迅速增加,但高浓度盐分胁迫后期叶片中的脯氨酸含量在品种间表现出显著差异;可溶性糖含量的变化在各种浓度盐分胁迫期间都表现出先升高后降低的趋势,但各个品种下降的时期不同;可溶性蛋白含量随盐分胁迫的持续不断降低,但湖桑32号和鲁插1号叶片中的可溶性蛋白含量在高浓度盐分胁迫后期略有升高.以上结果提示:在桑树对盐分胁迫的响应过程中,叶片中的渗透调节物质可能起到一定的调节作用,而不同品种间表现出的差异性可能与品种的抗盐能力有关.
以農桑14號、昂綠1號、湖桑32號、魯插1號4箇桑樹品種的2年生嫁接苗為材料,研究以不同濃度鹽分脅迫不同時間後桑樹葉片內滲透調節物質含量的變化.在鹽分脅迫下,4箇桑樹品種葉片中的脯氨痠含量及可溶性糖和可溶性蛋白含量的變化呈現一定規律性,且品種間具有顯著差異.4箇桑樹品種葉片中的脯氨痠含量在各種濃度鹽分脅迫初期都迅速增加,但高濃度鹽分脅迫後期葉片中的脯氨痠含量在品種間錶現齣顯著差異;可溶性糖含量的變化在各種濃度鹽分脅迫期間都錶現齣先升高後降低的趨勢,但各箇品種下降的時期不同;可溶性蛋白含量隨鹽分脅迫的持續不斷降低,但湖桑32號和魯插1號葉片中的可溶性蛋白含量在高濃度鹽分脅迫後期略有升高.以上結果提示:在桑樹對鹽分脅迫的響應過程中,葉片中的滲透調節物質可能起到一定的調節作用,而不同品種間錶現齣的差異性可能與品種的抗鹽能力有關.
이농상14호、앙록1호、호상32호、로삽1호4개상수품충적2년생가접묘위재료,연구이불동농도염분협박불동시간후상수협편내삼투조절물질함량적변화.재염분협박하,4개상수품충협편중적포안산함량급가용성당화가용성단백함량적변화정현일정규률성,차품충간구유현저차이.4개상수품충협편중적포안산함량재각충농도염분협박초기도신속증가,단고농도염분협박후기협편중적포안산함량재품충간표현출현저차이;가용성당함량적변화재각충농도염분협박기간도표현출선승고후강저적추세,단각개품충하강적시기불동;가용성단백함량수염분협박적지속불단강저,단호상32호화로삽1호협편중적가용성단백함량재고농도염분협박후기략유승고.이상결과제시:재상수대염분협박적향응과정중,협편중적삼투조절물질가능기도일정적조절작용,이불동품충간표현출적차이성가능여품충적항염능력유관.
In present study,two-year-old grafted mulberry saplings of 4 mulberry varieties namely Nongsang 1 2,Angl(u) 1.Husang 32 and Lucha 1 were used to investigate the effect of different salt stress on the contents of osmotic regulatory substances in mulberry leaves.The results showed that,under different salt stresses,changes of proline,soluble sugar and soluble protein contents in leaves of the 4 mulberry varieties had certain regularity and showed significant differences between different varieties.In the early stage of various salt stresses.proline contents in mulberry leaves of all 4 varieties increased rapidly and maintained at a high level.But significant differences between different varieties were only ob-served at the late stage of high salt stress.The soluble sugar contents increased in early stage of the stress and declinedin the late stage.Bul when to begin the decline was different across different varieties.The soluble protein contents de- clined along with the salt stress.However.the soluble protein contents in mulberry leaves of Husang 32 and Lucha 1 in-creased slightly in late stage of high salt stress.The above results indicate that,in response to salt stress,the osmotic regulatory substances in mulberry leaves have certain osmotic regulatory functions,and the response difference across the 4 varieties can be attributed to their varied tolerances to salt.