CAJ | 학술논문

目的研究非小细胞肺癌(NSCLC)组织中抑癌基因PTEN的表达和DNA含量与NSCLC侵袭、转移的关系.方法采用免疫组织化学SP方法检测PTEN在78例肺癌标本中的表达,并用流式细胞术检测30例肺癌标本中DNA含量.结果:肺癌标本中PTEN蛋白总缺失率为42.3%,有淋巴结转移组和无淋巴结转移组肺癌表达缺失率分别为52.1%和26.7%(P＜0.05),其表达缺失率随TNM分期增加而上升,分期越晚表达缺失率越高.PTEN缺失率高者生存时间短.DNA指数(DI)的分布范围在1.04～1.93.异倍体肿瘤24例,DI值随TNM分期增加而增加(P＜0.05),与淋巴结转移呈正相关.结论肺癌组织中PTEN的表达与肺癌淋巴结转移有显著相关性,肺癌细胞DNA含量与肺癌TNM分期及淋巴结转移密切相关.检测PTEN蛋白表达和DNA含量将有助于判断肺癌的转移及预后.
목적 연구비소세포폐암(NSCLC)조직중억암기인PTEN적표체화DNA함량여NSCLC침습、전이적관계.방법 채용면역조직화학SP방법검측PTEN재78례폐암표본중적표체,병용류식세포술검측30례폐암표본중DNA함량.결과:폐암표본중PTEN단백총결실솔위42.3%,유림파결전이조화무림파결전이조폐암표체결실솔분별위52.1%화26.7%(P＜0.05),기표체결실솔수TNM분기증가이상승,분기월만표체결실솔월고.PTEN결실솔고자생존시간단.DNA지수(DI)적분포범위재1.04～1.93.이배체종류24례,DI치수TNM분기증가이증가(P＜0.05),여림파결전이정정상관.결론 폐암조직중PTEN적표체여폐암림파결전이유현저상관성,폐암세포DNA함량여폐암TNM분기급림파결전이밀절상관.검측PTEN단백표체화DNA함량장유조우판단폐암적전이급예후.
Objective This study was designed to determine the expression ofgeneproducts of phosphatase and tensin homoligy deleted onehromoseten(PTEN)and DNA content in non small cell lung cancer(NSCLC)tissue and investigate their association with the invasion and metastasis of NSCLC.Methods The expression of PTEN were detected in 78 cases of lung cancer tissues by immunohistochemistry SP methods,DNA content in 30 cases of lung cancer tissues was examined by flow cytometry.Results The rate of total expression depletion of PTEN was 42.3%.The rates of PTEN expression depletion in lymph node metastasis group and no lymph node metastasis group were 52.1%and 26.7%respectively,with significantant difference(P＜0.05).The patients who had high expression of PTEN had a longer survival time.The range of the DNA index(DI)distribution ranged from 1.04 to 1.93,the DNA index and aneuploid tumor had a positive correlation with the lymph node metastasis,with the increased of TNM stage,the DI and the rate of DNA aneuploid increased(P＜0.05).Conclusions Expression of PTEN is associated strongly with lymph node metastasis of lung cancer,correlations exist between DNA content and TNM stage,lymph node metastasis.They are indications of metastasis potential and prognosis of lung cancer.