CAJ | 학술논문

目的探讨Akt在银屑病发病中的意义.方法免疫组化、免疫印迹法与活性测定的方法对30例寻常型银屑病患者(进行期皮损和非皮损表皮)和20例正常人表皮中Akt的表达与磷酸化水平及Akt的活性分别进行检测,免疫染色的强度进行光密度测定,免疫印迹和激酶活性测定结果均采朋灰度扫描.统计学处理采用方差分析和t检验.结果免疫组化显示:正常表皮、银屑病皮损和非皮损表皮内Akt蛋白的表达水平差异无统计学意义(Fakt=0.611,P＞0.05);正常表皮与银屑病非皮损表皮内,磷酸化Akt的表达差异无统计学意义(Tp-akt=0.624,P＞0.05),与二者相比,银屑病皮损表皮内磷酸化Akt的表达明显增强(Fp-akt=19.081,P＜0.01 o免疫印迹的结果(Takt=1.378,P＞0.05,Tp-akt=237.75,P＜0.01)与免疫组化一致.与正常表皮相比,银屑病皮损表皮内Akt的活性增强(Tp-akt=138.441,P＜0.01).结论银屑病角质形成细胞的过速增殖可能与银屑病皮损表皮内Akt的活性增强有关.
목적 탐토Akt재은설병발병중적의의.방법 면역조화、면역인적법여활성측정적방법대30례심상형은설병환자(진행기피손화비피손표피)화20례정상인표피중Akt적표체여린산화수평급Akt적활성분별진행검측,면역염색적강도진행광밀도측정,면역인적화격매활성측정결과균채붕회도소묘.통계학처리채용방차분석화t검험.결과 면역조화현시:정상표피、은설병피손화비피손표피내Akt단백적표체수평차이무통계학의의(Fakt=0.611,P＞0.05);정상표피여은설병비피손표피내,린산화Akt적표체차이무통계학의의(Tp-akt=0.624,P＞0.05),여이자상비,은설병피손표피내린산화Akt적표체명현증강(Fp-akt=19.081,P＜0.01 o면역인적적결과(Takt=1.378,P＞0.05,Tp-akt=237.75,P＜0.01)여면역조화일치.여정상표피상비,은설병피손표피내Akt적활성증강(Tp-akt=138.441,P＜0.01).결론 은설병각질형성세포적과속증식가능여은설병피손표피내Akt적활성증강유관.
Objective To investigate the significance of Akt in the pathogenesis of psoriasis.Methods Tissue specimens were obtained from involved and uninvolved skin of 30 patients with progressive psoriasis vulgaris and normal skin of 20 human controls.Immunohistochemistry.immunobloting and kinase activity assay were performed to detect the expressions of Akt and phosphorylated Akt as well as Akt activities in these specimens.Immunostaining intensity Was assessed by optical density detection and the results of immunobiot and activity assay by grey scanning.Statistical analyses were performed by variance analysis and student's t test.Results As immunohistochemistry revealed.there was no significant difierence in Akt protein expression among normal epidermis,psoriatic epidermis and uninvolved epidermis(F=0.611,P＞0.05):the level of phosphorylated Akt in psoriatic epidermis was significantly higher than that in normal epidermis and psoriatic uninvolved epidermis(F=19.081.P＜0.01).while no significant difierence was observed between normal epidermis and psoriatic uninvolved epidermis (t=0.624.P＞0.05).Immunoblot showed a significant difierence in phosphorylated Akt(t=237.75.P＜0.01)but not in Akt(t=1.378,P＞0.05)between psoriatic involved epidermis and normal epidermis.In comparison with normal epidermis,the activity of Akt in psoriatic involved epidermis was increased significantly(t=138.44 1.P＜0.0 1).Conclusion The overproliferation of psoriatic keratinocytcs may be associated with increased activation of Akt.