CAJ | 학술논문

目的研究神经轴突导向因子-1(Netrin-1)在体内和离体血管生成模型中的作用.方法通过离体大鼠动脉环实验和小鼠活体内Matrigel plug方法检测Netrin-1对血管形成的影响.结果 ①活体外大鼠动脉环实验结果表明10μg/L,50μg/L和100 μg/L Netrin-1均能够促进血管的生成,且50μg/L Netrin1具有最大效应.②小鼠活体内Matrigel plug结果显示含有Netrin-1的Matrigel plug变为红色,CD34免疫荧光染色显示Matrigel plug内有血管样结构形成,所含血红蛋白值为53.4±7.3,与空白对照组5.8±0.9比较差异有统计学意义(P＜0.01).结论 Netrin-1在在体和离体血管生成模型中均具有促血管生成效应,是一种有效的血管生成因子.
목적 연구신경축돌도향인자-1(Netrin-1)재체내화리체혈관생성모형중적작용.방법 통과리체대서동맥배실험화소서활체내Matrigel plug방법검측Netrin-1대혈관형성적영향.결과 ①활체외대서동맥배실험결과표명10μg/L,50μg/L화100 μg/L Netrin-1균능구촉진혈관적생성,차50μg/L Netrin1구유최대효응.②소서활체내Matrigel plug결과현시함유Netrin-1적Matrigel plug변위홍색,CD34면역형광염색현시Matrigel plug내유혈관양결구형성,소함혈홍단백치위53.4±7.3,여공백대조조5.8±0.9비교차이유통계학의의(P＜0.01).결론 Netrin-1재재체화리체혈관생성모형중균구유촉혈관생성효응,시일충유효적혈관생성인자.
Objective To investigate the effects of Netrin-1 on angiogenesis in vitro and in vivo. Methods We performed in vitro rat aortic ring assay and in vivo Matrigel plug assay to determine the effect of Netrin-1 on angiogenesis. Results 10 μg/L, 50 μg/L and 100 μg/L Netrin-1 stimulated microvessel sprouting from the adventitia of aortic rings and the effect reached maximum at 50 μg/L The in vivo Matrigel plug assay showed orange color change if Nestrin-1 was positive; and CD34 immunofluorescent staining showed vascular structures in the Matrigel plug with hemachrome ( 53.4 ± 7. 3 ), which was significantly higher than control ( 5. 8 ± 0. 9 )Conclusions Netrin-1 can induce angiogenesis in vitro and in vivo.