中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2011年
5期
394-396
,共3页
程继文%武有祯%聂继盛%牛侨
程繼文%武有禎%聶繼盛%牛僑
정계문%무유정%섭계성%우교
苯并(a)芘%学习记忆%海马%N-甲基-D天门冬氨酸受体NR2B亚基%脑源性生长因子
苯併(a)芘%學習記憶%海馬%N-甲基-D天門鼕氨痠受體NR2B亞基%腦源性生長因子
분병(a)비%학습기억%해마%N-갑기-D천문동안산수체NR2B아기%뇌원성생장인자
Benzo(a)pyrene%Learning and memory%Hippocampus%NMDAR2B%Brain-derived neurotrophic factor
目的 探讨不同剂量苯并(a)芘[B(a)P]对断乳大鼠学习记忆能力的影响及其机制.方法 将40只SD断乳大鼠(28d)随机分为5组,空白对照组、溶剂对照组、3个染毒组(浓度分别为5,10和20mg/kg体质量),隔日腹腔染毒30d.染毒结束后用Morris水迷宫试验观察,免疫组化法测定海马组织中N-甲基-D天门冬氨酸受体NR2B亚基(NMDAR2B)和脑源性生长因子(BDNF)含量.结果 Morris水迷宫试验结果显示,随B(a)P染毒剂量的增加,逃避潜伏期呈递减趋势,高剂量组[(62.78 ±47.25)s]与对照组[(40.60 4-38.79)s]相比,差异有统计学意义(P<0.01);跨台次数随染毒剂量的增加逐渐减少,高剂量组[(4.33 ± 2.08)次]与对照组[(11.25 ± 2.63)次]相比.差异有统计学意义(P＜0.05);随染毒剂量增加,海马区NMDAR2B灰度值呈递减趋势,高剂量组(150.38 ± 15.34)与对照组(162.23±6.56)相比,差异有统计学意义(P＜0.05);BDNF灰度值除低剂量组外呈递减趋势,高剂量组(141.83 ±13.37)与对照组(163.13±8.09)、低剂量组(164.56±9.10)相比均差异有统计学意义(P＜0.05).结论 亚急性B(a)P暴露可降低断乳大鼠空间学习记忆能力,其机制可能与B(a)P影响海马NMDAR2B和BDNF的表达有关.
目的 探討不同劑量苯併(a)芘[B(a)P]對斷乳大鼠學習記憶能力的影響及其機製.方法 將40隻SD斷乳大鼠(28d)隨機分為5組,空白對照組、溶劑對照組、3箇染毒組(濃度分彆為5,10和20mg/kg體質量),隔日腹腔染毒30d.染毒結束後用Morris水迷宮試驗觀察,免疫組化法測定海馬組織中N-甲基-D天門鼕氨痠受體NR2B亞基(NMDAR2B)和腦源性生長因子(BDNF)含量.結果 Morris水迷宮試驗結果顯示,隨B(a)P染毒劑量的增加,逃避潛伏期呈遞減趨勢,高劑量組[(62.78 ±47.25)s]與對照組[(40.60 4-38.79)s]相比,差異有統計學意義(P<0.01);跨檯次數隨染毒劑量的增加逐漸減少,高劑量組[(4.33 ± 2.08)次]與對照組[(11.25 ± 2.63)次]相比.差異有統計學意義(P＜0.05);隨染毒劑量增加,海馬區NMDAR2B灰度值呈遞減趨勢,高劑量組(150.38 ± 15.34)與對照組(162.23±6.56)相比,差異有統計學意義(P＜0.05);BDNF灰度值除低劑量組外呈遞減趨勢,高劑量組(141.83 ±13.37)與對照組(163.13±8.09)、低劑量組(164.56±9.10)相比均差異有統計學意義(P＜0.05).結論 亞急性B(a)P暴露可降低斷乳大鼠空間學習記憶能力,其機製可能與B(a)P影響海馬NMDAR2B和BDNF的錶達有關.
목적 탐토불동제량분병(a)비[B(a)P]대단유대서학습기억능력적영향급기궤제.방법 장40지SD단유대서(28d)수궤분위5조,공백대조조、용제대조조、3개염독조(농도분별위5,10화20mg/kg체질량),격일복강염독30d.염독결속후용Morris수미궁시험관찰,면역조화법측정해마조직중N-갑기-D천문동안산수체NR2B아기(NMDAR2B)화뇌원성생장인자(BDNF)함량.결과 Morris수미궁시험결과현시,수B(a)P염독제량적증가,도피잠복기정체감추세,고제량조[(62.78 ±47.25)s]여대조조[(40.60 4-38.79)s]상비,차이유통계학의의(P<0.01);과태차수수염독제량적증가축점감소,고제량조[(4.33 ± 2.08)차]여대조조[(11.25 ± 2.63)차]상비.차이유통계학의의(P＜0.05);수염독제량증가,해마구NMDAR2B회도치정체감추세,고제량조(150.38 ± 15.34)여대조조(162.23±6.56)상비,차이유통계학의의(P＜0.05);BDNF회도치제저제량조외정체감추세,고제량조(141.83 ±13.37)여대조조(163.13±8.09)、저제량조(164.56±9.10)상비균차이유통계학의의(P＜0.05).결론 아급성B(a)P폭로가강저단유대서공간학습기억능력,기궤제가능여B(a)P영향해마NMDAR2B화BDNF적표체유관.
Objective To investigate the changes and mechanism of learning and memory in rats by different doses of benzo (a) pyrene (B(a)P). Methods Forty weaned rats (28 days) were randomly divided into control group (NS), solvent group ( vegetable oil) and three B (a) P dosage groups (the doses were 5,10 and 20 mg / kg body weight respectively ). And all rats were administrated intraperitoneally every other day to one month. The capability of learning and memory in rats were measured by Morris water maze test, and the brain-derived neurotrophic factor ( BDNF) and NMDAR2B content in hippocampus were tested by immunohistochemistry. Results In training of Morris water maze,the average escape latency was extended gradually with increasing dose, and there was a statistically significant difference between high-dose group((62. 78 ±47. 25 )s) and the control group((40.60±38.79)s)(P＜ 0.01). Compared with the control group(11.25 ±2.63), the number of crossplatform of high-dose group(4.33 ±2.08) was statistically reduced (P＜0.05). B(a)P at 10 and 20 mg/kg decreased NMDAR2B and BDNF expression in hippocampus of rats in immunohistochemistry. The level of NMDAR2B was (162.23 ±6.56) in the high-dose group and (150.38 ± 15.34) in the control group(P＜0.05);the expression level of BDNF was (163. 13 ± 8.09) in the high-dose group and (141.83 ± 13.37) in the control group(P＜ 0.05). Conclusion Subacute B(a)P exposure can reduce spatial learning and memory in weaning rats, it may be related to decreased levels of NMDAR2B and BDNF in hippocampus.
