CAJ | 학술논문

目的研究吡虫啉体外经皮渗透吸收的效果,分析样品浓度和皮肤蓄积作用等对农药风险评估的影响.方法采用Franz垂直扩散池,以雄性Wistar大鼠离体腹部新鲜皮肤或冷冻皮肤为渗透屏障,C/MS/MS法测定接受液和皮肤中的吡虫啉含量,分别比较高低两种吡虫啉溶液和两类皮肤的透皮效果.结果在使用新鲜皮肤的体外试验中,高、低两种浓度的接受液中吡虫啉含量占总受试物的比例均数分别为6.8％,6.6％,皮肤残留中吡虫啉含量占总受试物的比例均数分别为10.7％,1.3％,经皮渗透吸收率为分别17.5％和7.9％.在使用同种(低浓度)浓度新鲜和冷冻皮肤的体外试验中,新鲜皮肤和冷冻皮肤的接受液中吡虫啉含量占总受试物的比例均数分别为6.6％,0.7％,皮肤残留中吡虫啉含量占总受试物的比例均数分别为1.3％,10.7％,经皮渗透吸收率分别为7.9％和11.4％.结论高浓度吡虫啉在新鲜皮肤上的经皮渗透吸收率最大,是本实验吡虫啉暴露“最坏情况”的估计值,也是可用于风险评估的参考值.
목적 연구필충람체외경피삼투흡수적효과,분석양품농도화피부축적작용등대농약풍험평고적영향.방법 채용Franz수직확산지,이웅성Wistar대서리체복부신선피부혹냉동피부위삼투병장,C/MS/MS법측정접수액화피부중적필충람함량,분별비교고저량충필충람용액화량류피부적투피효과.결과 재사용신선피부적체외시험중,고、저량충농도적접수액중필충람함량점총수시물적비례균수분별위6.8％,6.6％,피부잔류중필충람함량점총수시물적비례균수분별위10.7％,1.3％,경피삼투흡수솔위분별17.5％화7.9％.재사용동충(저농도)농도신선화냉동피부적체외시험중,신선피부화냉동피부적접수액중필충람함량점총수시물적비례균수분별위6.6％,0.7％,피부잔류중필충람함량점총수시물적비례균수분별위1.3％,10.7％,경피삼투흡수솔분별위7.9％화11.4％.결론 고농도필충람재신선피부상적경피삼투흡수솔최대,시본실험필충람폭로“최배정황”적고계치,야시가용우풍험평고적삼고치.
Objective The dermal absorption of Imidacloprid was studied to understand the effects of concentrations and skin reservoir on pesticide risk assessment in in vitro absorption studies.Methods By using Franz diffusion cell and the transdermal barrier of viable Wistar rat abdomen skin or frozen ones,the imidacloprid content in the receptor fluid and skin was determined by LC/MS/MS method,and the absorption effects were compared between two concentrations of Imidacloprid solutions and two types of skin,respectively.Results All percentages reported are ％ of applied dose.In vitro studies using viable skin,the Imidacloprid content in the receptor fluid of high and low concentration was 6.8％,6.6％ respectively; and 10.7％,1.3％ in skin,thus total absorption was 17.5％ and 7.9％.And in vitro studies using both viable and frozen skin under the same concentration circumstances,the Imidacloprid content in the receptor fluid of viable and frozen skin was 6.6％ and 0.7％ respectively,in skin was 1.3％ and 10.7％,and total absorption was 7.9％ and 11.4％.Conclusion Comparison of these in vitro results showed that either concentrations or skin reservoir had an effect on the dermal absorption.During 6h exposure,the high concentration in viable skin had the maximum dermal absorption value,which was the worst-case exposure estimate,also the best single estimate for pesticide risk assessment.