中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2010年
4期
370-373
,共4页
生殖细胞肿瘤%p53基因%mdm2基因
生殖細胞腫瘤%p53基因%mdm2基因
생식세포종류%p53기인%mdm2기인
Germ cell tumor,p53 gene%mdm2 gene
目的 研究鼠双微基因2(mdm2)基因和p53基因在颅内非生殖细胞瘤性生殖细胞肿瘤患者(NGGCTs)中的表达及意义. 方法 利用半定量RT-PCR技术检测了15例NGGCTs肿瘤(成熟畸胎瘤6例,未成熟畸胎瘤5例,卵黄囊瘤2例,绒毛膜上皮癌2例)中mdm2和p53 mRNA的表达,并与正常人群进行比较,另外检测p53基因5～8外显子的突变情况.结果 p53和mdm2mRNA在各类NGGCTs中均有较高水平表达,与正常对照组比较差异有统计学意义(P=0.000);p53mRNA在各类肿瘤中表达强度差异无统计学意义(P=0.056);mdm2基因在非生殖细胞瘤性恶性生殖细胞肿瘤(NGMGCTs)中表达强度较成熟畸胎瘤高,差异有统计学意义(P=0.000);mdm2和p53mRNA的表达强度无显著相关性(r=0.418,P=0.121);15例NGGCTs肿瘤均未检测到p53基因的突变.结论 mdm2基因异常表达与NGMGCTs肿瘤关系密切,p53基因突变并非NGGCTs形成的主要原因,mdm2和p53之间相互作用共同影响NGGCTs肿瘤的发生发展过程.
目的 研究鼠雙微基因2(mdm2)基因和p53基因在顱內非生殖細胞瘤性生殖細胞腫瘤患者(NGGCTs)中的錶達及意義. 方法 利用半定量RT-PCR技術檢測瞭15例NGGCTs腫瘤(成熟畸胎瘤6例,未成熟畸胎瘤5例,卵黃囊瘤2例,絨毛膜上皮癌2例)中mdm2和p53 mRNA的錶達,併與正常人群進行比較,另外檢測p53基因5～8外顯子的突變情況.結果 p53和mdm2mRNA在各類NGGCTs中均有較高水平錶達,與正常對照組比較差異有統計學意義(P=0.000);p53mRNA在各類腫瘤中錶達彊度差異無統計學意義(P=0.056);mdm2基因在非生殖細胞瘤性噁性生殖細胞腫瘤(NGMGCTs)中錶達彊度較成熟畸胎瘤高,差異有統計學意義(P=0.000);mdm2和p53mRNA的錶達彊度無顯著相關性(r=0.418,P=0.121);15例NGGCTs腫瘤均未檢測到p53基因的突變.結論 mdm2基因異常錶達與NGMGCTs腫瘤關繫密切,p53基因突變併非NGGCTs形成的主要原因,mdm2和p53之間相互作用共同影響NGGCTs腫瘤的髮生髮展過程.
목적 연구서쌍미기인2(mdm2)기인화p53기인재로내비생식세포류성생식세포종류환자(NGGCTs)중적표체급의의. 방법 이용반정량RT-PCR기술검측료15례NGGCTs종류(성숙기태류6례,미성숙기태류5례,란황낭류2례,융모막상피암2례)중mdm2화p53 mRNA적표체,병여정상인군진행비교,령외검측p53기인5～8외현자적돌변정황.결과 p53화mdm2mRNA재각류NGGCTs중균유교고수평표체,여정상대조조비교차이유통계학의의(P=0.000);p53mRNA재각류종류중표체강도차이무통계학의의(P=0.056);mdm2기인재비생식세포류성악성생식세포종류(NGMGCTs)중표체강도교성숙기태류고,차이유통계학의의(P=0.000);mdm2화p53mRNA적표체강도무현저상관성(r=0.418,P=0.121);15례NGGCTs종류균미검측도p53기인적돌변.결론 mdm2기인이상표체여NGMGCTs종류관계밀절,p53기인돌변병비NGGCTs형성적주요원인,mdm2화p53지간상호작용공동영향NGGCTs종류적발생발전과정.
Objective To explore the significance of abnormal expressions of p53 and mdm2 genes in the intracranial nongerminomatous germ cell tumors (NGGCTs). Methods Fifteen patients with NGGCTs, including 6 with mature teratoma, 5 with immature teratoma, 2 with Yolk sac tumor and 2 with choriocarcinoma, were selected; semiquantitative RT-PCR was employed to observe their mRNA expressions of mdm2 and p53 genes; the p53 gene mutation in exon 5-8 was also detected. Results High-level mRNA expressions of p53 and mdm2 genes in patients with NGMGCTs were observed as compared with those in the normal group (P=0.000). The mRNA expression of the P53 gene was not statistically different among various sub-kinds of tumors (P=0.056). Significantly high mRNA expression of mdm2 gene in patients with NGMGCTs was noted than that in patients with mature teratoma (P=0.000). No significant relation was observed between mRNA expressions of mdm2 and p53 genes (r=0.418, P=0.121) and no p53 gene mutation was found in all the patients. Conclusions The abnormal expression of mdm2 gene might relate to the development of NGMGCTs and p53 gene mutation might not be the main cause of the oncogenesis of NGGCTs. The interaction of mdm2 and p53 genes plays a promotive role in the oncogenesis and development of NGGCTs.
