CAJ | 학술논문

目的探讨促红细胞生成素(EPO)后处理对家兔蛛网膜下腔出血(SAH)后脑血管痉挛的逆转作用.方法 30只新西兰白兔按照随机数字表法分为5组:Sham组、SAH0组、SAH1组、SAH2组、SAH3组.Sham组假穿刺,其他4组行枕大池穿刺注血的方法造模.Sham组和SAH0组造模24 h后静脉单次注入生理盐水0.1 mL/kg,SAH1组、SAH2组、SAH3组分别静脉单次注入EPO 500 IU/kg、1000 IU/kg、2000 IU/kg.所有动物存造模完成后48 h处死,使用Image Pro Plus 6.0图像分析系统测量分析并比较不同组问基底动脉管腔面积、基底动脉收缩系数以及海马CAI区神经元密度.结果基底动脉形态学观察结果可见Sham组血管管壁无增厚、无增生或坏死:SAH0组、SAH1组血管壁明显增厚,结构紊乱,中膜明显变厚,平滑肌排列紊乱;SAH3组血管内弹力膜皱折,SAH2组血管改变介于SAH1和SAH3组之间.基底动脉管腔面积SAH2组[(0.10±0.01)mm2]、SAH3组[(0.16±0.02)mm2]较SAH0组[(0.07±0.02)mm2]明显增大,基底动脉收缩系数SAH2组(1.22±0.06)、SAH3组(1.15±0.03)较SAH0组(1.31±0.09)明显减小,海马神经元密度SAH3组[(126.8±5.7)个/mml较SAH0组[(99.3±9.6)个/mm]明显增大,差异均有统计学意义(p<0.05).结论在SAH后24h,单次静脉注射EPO不仅可以逆转家兔基底动脉痉挛,还可以减轻其脑神经细胞损伤.
목적 탐토촉홍세포생성소(EPO)후처리대가토주망막하강출혈(SAH)후뇌혈관경련적역전작용.방법 30지신서란백토안조수궤수자표법분위5조:Sham조、SAH0조、SAH1조、SAH2조、SAH3조.Sham조가천자,기타4조행침대지천자주혈적방법조모.Sham조화SAH0조조모24 h후정맥단차주입생리염수0.1 mL/kg,SAH1조、SAH2조、SAH3조분별정맥단차주입EPO 500 IU/kg、1000 IU/kg、2000 IU/kg.소유동물존조모완성후48 h처사,사용Image Pro Plus 6.0도상분석계통측량분석병비교불동조문기저동맥관강면적、기저동맥수축계수이급해마CAI구신경원밀도.결과기저동맥형태학관찰결과가견Sham조혈관관벽무증후、무증생혹배사:SAH0조、SAH1조혈관벽명현증후,결구문란,중막명현변후,평활기배렬문란;SAH3조혈관내탄력막추절,SAH2조혈관개변개우SAH1화SAH3조지간.기저동맥관강면적SAH2조[(0.10±0.01)mm2]、SAH3조[(0.16±0.02)mm2]교SAH0조[(0.07±0.02)mm2]명현증대,기저동맥수축계수SAH2조(1.22±0.06)、SAH3조(1.15±0.03)교SAH0조(1.31±0.09)명현감소,해마신경원밀도SAH3조[(126.8±5.7)개/mml교SAH0조[(99.3±9.6)개/mm]명현증대,차이균유통계학의의(p<0.05).결론 재SAH후24h,단차정맥주사EPO불부가이역전가토기저동맥경련,환가이감경기뇌신경세포손상.
Objective To investigate the reversal effect of intravenous injection of erythropoietin on vasospasm in the basilar artery in rabbits subjected to subarachnoid hemorrhage (SAH). Methods Thirty male New Zealand adult white rabbits weighing 2.0-2.5 kg were equally randomized into 5 groups: sham-operated group, SAH0 group, SAH1 group, SAH2 group and SAH3 group. SAH models of single-hemorrhage were established by injecting autologous arterial blood (1.0 mL/kg) into the cistema magna in SAH group's rabbits, while animals in the sham-operated group received an injection of normal saline (1.0 mlAg). Twenty-four h after SAH, rabbits in the sham-operated group and the SAHO group were received intravenous injection of normal saline (0.1 mL/kg), meanwhile, intravenous injection of r-Hu-EPO at dosages of 500,1000, or 2000 IU/kg was initiated in the SAH1 group, SAH2 group SAH3 group, respectively. All animals were sacrificed by perfusion-fixation 48 h after SAH induction. The cross section areas of basilar arteries, corrugation coefficient (CC) of the basilar arteries, and hippocampus normal neuron density of CA1 area were measured by Image-Pro-Plus software. Results Morphology observation on the basilar arteries showed no obvious changes in the sham-operated group, significantly thickened and disorganized vascular walls with thickened tunica media and disorganized smooth muscle in the SAH0 and SAH1 groups; the internal elastic lamina in the SAH3 group was folded. Compared with that in SAH0 group [(0.07±0.02) mm2], the cross-sectional area of basilar arteries in SAH2 [(0.10±0.01) mm2] and SAH3 [(0.16±0.02) mm2] groups were significantly increased (P<0.05); the CC in SAH2 (1.22± 0.06) and SAH3 (1.15±0.03) groups was significantly lower than that in the SAHO group (1.31±0.09, P<0.05); and the normal neural density of hippocampus in CA1 area in the SAH3 group [(126.8±5.7) cell/mm] was significantly higher than that in the SAHO group [(99.3±9.6) cell/mm, P<0.05]. Conclusion A single intravenous injection of erythropoietin 24 h after SAH can still reverse SAH-induced vasospasm and attenuate injury of neurons.