中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2011年
4期
520-522
,共3页
李世康%栗振坤%龙村%周琦云%冼磊%何巍
李世康%慄振坤%龍村%週琦雲%冼磊%何巍
리세강%률진곤%룡촌%주기운%승뢰%하외
吸烟%大隐静脉%内皮功能%冠状动脉旁路移植术
吸煙%大隱靜脈%內皮功能%冠狀動脈徬路移植術
흡연%대은정맥%내피공능%관상동맥방로이식술
Smoking%Great saphenous vein%Endothelium function%CABG
目的 观察四氢生物蝶呤(BH4)对吸烟者大隐静脉桥内皮功能的影响.方法 取12例吸烟(实验组)及10例不吸烟(对照组)行冠状动脉旁路移植术患者的大隐静脉.在器官槽中观察经重碳酸盐缓冲液(KH液)、含0.1 mol/L N-硝基-L-精氨酸(L-NNA)或含20 μmol/L BH4的KH液浸泡30 min后,苯肾上腺素(1×10-5 mol/L)及不同浓度(1×10-9～1×10-5mol/L)的非受体介导钙离子载体引发的血管舒缩反应.结果 最大收缩强度各组间差异无统计学意义(P＞0.05).最大舒张反应:经KH液浸泡后实验组低于对照组[(29.50±6.22)%比(48.20±7.74)%,P＜0.01];经L-NNA[(24.50±5.72)%比(28.80±5.64)%]或BH4[(46.40±6.32)%比(50.80±6.91)%]浸泡后两组差异无统计学意义(P＞0.05).结论 吸烟损伤大隐静脉桥内皮源性一氧化氮介导的血管舒张功能,BH4有利于此功能的恢复.
目的 觀察四氫生物蝶呤(BH4)對吸煙者大隱靜脈橋內皮功能的影響.方法 取12例吸煙(實驗組)及10例不吸煙(對照組)行冠狀動脈徬路移植術患者的大隱靜脈.在器官槽中觀察經重碳痠鹽緩遲液(KH液)、含0.1 mol/L N-硝基-L-精氨痠(L-NNA)或含20 μmol/L BH4的KH液浸泡30 min後,苯腎上腺素(1×10-5 mol/L)及不同濃度(1×10-9～1×10-5mol/L)的非受體介導鈣離子載體引髮的血管舒縮反應.結果 最大收縮彊度各組間差異無統計學意義(P＞0.05).最大舒張反應:經KH液浸泡後實驗組低于對照組[(29.50±6.22)%比(48.20±7.74)%,P＜0.01];經L-NNA[(24.50±5.72)%比(28.80±5.64)%]或BH4[(46.40±6.32)%比(50.80±6.91)%]浸泡後兩組差異無統計學意義(P＞0.05).結論 吸煙損傷大隱靜脈橋內皮源性一氧化氮介導的血管舒張功能,BH4有利于此功能的恢複.
목적 관찰사경생물접령(BH4)대흡연자대은정맥교내피공능적영향.방법 취12례흡연(실험조)급10례불흡연(대조조)행관상동맥방로이식술환자적대은정맥.재기관조중관찰경중탄산염완충액(KH액)、함0.1 mol/L N-초기-L-정안산(L-NNA)혹함20 μmol/L BH4적KH액침포30 min후,분신상선소(1×10-5 mol/L)급불동농도(1×10-9～1×10-5mol/L)적비수체개도개리자재체인발적혈관서축반응.결과 최대수축강도각조간차이무통계학의의(P＞0.05).최대서장반응:경KH액침포후실험조저우대조조[(29.50±6.22)%비(48.20±7.74)%,P＜0.01];경L-NNA[(24.50±5.72)%비(28.80±5.64)%]혹BH4[(46.40±6.32)%비(50.80±6.91)%]침포후량조차이무통계학의의(P＞0.05).결론 흡연손상대은정맥교내피원성일양화담개도적혈관서장공능,BH4유리우차공능적회복.
Objective To observe the effect of tetrahydrobiopterin ( BH4 ) on endothelium function of the great saphenous vein from smokers. Methods The rings of great saphenous vein were selected from 22 patients with coronary artery bypass grafting, which including smoking (experimental group,n = 12) and non-smoking (control group,n = 10) patients. After incubation in Krebs-Henseleit (KH), KH plus BH4 (20 μ mol/L) or N-nitro-L-arginine ( L-NNA, 0. 1 mol/L), the vasoconstriction and vasodilatation were induced by phenylephrine (1 × 10-5 mol/L) and non-receptor-mediated calcium ionophore (A23187,1 × 10-9-1 × 10 -5 mol/L) separately in an organ chamber. Results The maximum constriction in all groups had no significant difference ( P ＞ 0. 05 ). The maximum relaxation was significantly decreased in experimental group as compared with control group [(29.50 ± 6. 22 ) % vs (48.20 ± 7. 74 ) %, P ＜ 0. 01]after incubation in KH, while there was no significant difference (P ＞ 0. 05 ) after incubation in KH plus L-NNA [(24.50±5.72)% vs (28.8 ±5.64)%] or BH4[(46.40 ±6.32)% vs (50.80±6.91)%].Conclusion Smoking impairs the relaxation function mediated by endothelium-derived nitric oxide in great saphenous, which may be restored by BH4.
