CAJ | 학술논문

目的探讨微小RNA前体(pre-miRNA)区域基因多态与中国人群肝细胞肝癌(hepatocellular carcinoma,HCC)易感性的关系.方法采用病例-对照研究设计,包括确诊的HCC患者963例、乙型肝炎病毒(hepatitis B virus,HBV)阳性对照829例和HBV阴性对照852名.选取pre-miRNA区域多态位点hsa-mir-146a rs2910164 C→G及hsa-mir-196-a2 rs11614913 T→C为研究位点,应用引物错配限制性分析(primer introduced restriction analysis-PCR,PIRA-PCR)方法进行多态性检测,应用logistic回归计算OR值及95%CI,比较不同基因型与HCC发病风险的关系.结果 rs2910164位点3种基因型CC、CG、GG在病例组分布频率分别为34.5%(319/925)、48.6%(450/925)、16.9%(156/925),在HBV阳性对照组中分别为36.4%(274/753)、45.0%(339/753)、18.6%(140/753),在HBV阴性对照组中分别为36.1%(303/840)、46.0%(386/840)、18.0%(151/840).rs11614913位点3种基因型TT、CT、CC在病例组分布频率分别为29.7%(277/934)、48.1%(449/934)、22.3%(208/934),在HBV阳性对照组中分别为30.3%(238/785)、51.0%(400/785)、18.7%(147/785),在HBV阴性对照组中分别为28.6%(239/837)、49.8%(417/837)、21.6%(181/837).在调整年龄、性别、吸烟、饮酒因素后,未能发现两位点多态与HCC发病危险之间存在明显关联[与HBV阳性对照相比:hsa-mir-146a rs2910164(GC+GG对CC):校正0R=1.10,95%CI:0.90～1.36;hsa-mir-196-a2 rs11614913(CC+CT对TT):校正OR=1.01,95%CI:0.81～1. 25;与HBV阴性对照相比:hsa-mir-146a rs2910164(GC+GG对CC):校正OR=1.06,95%CI:0.87～1.29;hsa-mir-196-a2 rs11614913(CC+CT对TT):校正OR=0.94,95%CI:0.76～1.16].分别以年龄、性别、吸烟、饮酒进行分层分析也未能发现两多态位点与HCC发病风险相关联.结论 hsa-mir-146a rs2910164 C→G及hsa-mir-196-a2 rs11614913 T→C多态性可能不是中国人群HCC的易感性标志物.
목적 탐토미소RNA전체(pre-miRNA)구역기인다태여중국인군간세포간암(hepatocellular carcinoma,HCC)역감성적관계.방법 채용병례-대조연구설계,포괄학진적HCC환자963례、을형간염병독(hepatitis B virus,HBV)양성대조829례화HBV음성대조852명.선취pre-miRNA구역다태위점hsa-mir-146a rs2910164 C→G급hsa-mir-196-a2 rs11614913 T→C위연구위점,응용인물착배한제성분석(primer introduced restriction analysis-PCR,PIRA-PCR)방법진행다태성검측,응용logistic회귀계산OR치급95%CI,비교불동기인형여HCC발병풍험적관계.결과 rs2910164위점3충기인형CC、CG、GG재병례조분포빈솔분별위34.5%(319/925)、48.6%(450/925)、16.9%(156/925),재HBV양성대조조중분별위36.4%(274/753)、45.0%(339/753)、18.6%(140/753),재HBV음성대조조중분별위36.1%(303/840)、46.0%(386/840)、18.0%(151/840).rs11614913위점3충기인형TT、CT、CC재병례조분포빈솔분별위29.7%(277/934)、48.1%(449/934)、22.3%(208/934),재HBV양성대조조중분별위30.3%(238/785)、51.0%(400/785)、18.7%(147/785),재HBV음성대조조중분별위28.6%(239/837)、49.8%(417/837)、21.6%(181/837).재조정년령、성별、흡연、음주인소후,미능발현량위점다태여HCC발병위험지간존재명현관련[여HBV양성대조상비:hsa-mir-146a rs2910164(GC+GG대CC):교정0R=1.10,95%CI:0.90～1.36;hsa-mir-196-a2 rs11614913(CC+CT대TT):교정OR=1.01,95%CI:0.81～1. 25;여HBV음성대조상비:hsa-mir-146a rs2910164(GC+GG대CC):교정OR=1.06,95%CI:0.87～1.29;hsa-mir-196-a2 rs11614913(CC+CT대TT):교정OR=0.94,95%CI:0.76～1.16].분별이년령、성별、흡연、음주진행분층분석야미능발현량다태위점여HCC발병풍험상관련.결론 hsa-mir-146a rs2910164 C→G급hsa-mir-196-a2 rs11614913 T→C다태성가능불시중국인군HCC적역감성표지물.
Objective To investigate the relationship between genetic polymorphism in microRNAs (miRNAs) precursor and genetic prediposition of hepatocellular carcinoma (HCC) in Chinese population. Methods A case-control study including 963 HCC cases and 829 HBsAg positive controls and 852 HBsAg negative controls was conducted. hsa-mir-146a rs2910164 C→G and hsa-mir-196-a2 rs11614913 T→C were selected,where the genotypes were determined by the primer introduced restriction analysis-PCR (PIRA-PCR) assay. Odd ratios (ORs) and 95% confidence intervals (CIs) were evaluated by logistic regression analysis to investigate the relationship between onset risk of HCC and different genotypes. Results The genotype frequencies of CC, CG and GG at rs2910164 gene locus were separately 34. 5 % ( 319/925 ) ,48.6% (450/925) and 16.9% ( 156/925 ) in cases; 36.4% ( 274/753 ) ,45.0% ( 339/753 ) and 18. 6%(140/753) in HBsAg positive controls; and 36. 1% (303/840) ,46.0% (386/840) and 18.0% (151/840) in HBsAg negative controls. The genotype frequencies of TT, CT and CC at rs11614913 were respectively 29.7% (277/934) ,48. 1% (449/934) and 22.3% (208/934) in cases; 30. 3% (238/785) ,51.0% (400/785) and 18. 7% (147/785) in HBsAg positive controls; and 28. 6% (239/837) ,49. 8% (417/837) and 21.6% ( 181/837 ) in HBsAg negative controls. No significant relationships were observed between these two single nucleotide polymorphisms (SNPs) and onset risk of HCC after adjusting the factors as age,gender,smoking and drinking status in comparison with HBsAg positive controls: hsa-mir-146a rs2910164 (CG +GG vs CC): adjusting OR = 1.10,95% CI: 0. 90 - 1.36; hsa-mir-196-a2 rs11614913 ( CC + CT vs TT):adjusting OR= 1.01,95% CI: 0. 81 -1.25; as well as in comparison with HBsAg negative controls: hsa-mir-146a rs2910164 ( CG + GG vs CC ): adjusting OR = 1. 06,95% CI: 0. 87 - 1.29; hsa-mir-196-a2 rs11614913 ( CC + CT vs TT ): adjusting OR = 0. 94,95% CI: 0. 76 - 1.16. As well, no significant relationships were observed between these two SNPs and onset risk of HCC in the subgroups stratified by age,gender,smoking and drinking status. Conclusion hsa-mir-146a rs2910164 C→G and hsa-mir-196-a2 rs11614913 T→C may not play an important role in the HCC predisposition among Chinese populations.