中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2012年
2期
172-177
,共6页
腺病毒5型%腺病毒7型%呼吸道上皮细胞%黏蛋白1%炎症调控
腺病毒5型%腺病毒7型%呼吸道上皮細胞%黏蛋白1%炎癥調控
선병독5형%선병독7형%호흡도상피세포%점단백1%염증조공
Adenovirus serotype 5%Adenovirus serotype 7%Airway epithelial cells%Mucin 1 ( MUC1 )%Inflammatory regulation
目的 为探讨人类呼吸道腺病毒感染自限性的分子机制,对人类常见呼吸道腺病毒5型(Ad5)和7型(Ad7)感染对呼吸道上皮细胞抑炎分子——黏蛋白1(MUC1)表达的影响进行初步研究,并探讨两者差异性.方法 分别用Ad7和Ad5感染呼吸道上皮细胞A549构建感染模型.qRT-PCR和Western blot分别检测感染后MUC1 mRNA转录水平及蛋白表达水平变化.结果 Ad5感染A549细胞后MUC1 mRNA转录水平及MUC1蛋白表达水平均可见上调,且呈一定的时间效应;而Ad7感染后未观察到此现象,延长Ad7感染时间后,仍未观察到MUC1 mRNA转录上调.结论 人类呼吸道Ad5感染呼吸道上皮细胞初期,可诱导上调抑炎分子MUC1表达,提示MUC1全部或部分参与到Ad5感染的自限性过程.但Ad7感染不能诱导上调MUC1表达,其感染的自限性来源于其他机制.
目的 為探討人類呼吸道腺病毒感染自限性的分子機製,對人類常見呼吸道腺病毒5型(Ad5)和7型(Ad7)感染對呼吸道上皮細胞抑炎分子——黏蛋白1(MUC1)錶達的影響進行初步研究,併探討兩者差異性.方法 分彆用Ad7和Ad5感染呼吸道上皮細胞A549構建感染模型.qRT-PCR和Western blot分彆檢測感染後MUC1 mRNA轉錄水平及蛋白錶達水平變化.結果 Ad5感染A549細胞後MUC1 mRNA轉錄水平及MUC1蛋白錶達水平均可見上調,且呈一定的時間效應;而Ad7感染後未觀察到此現象,延長Ad7感染時間後,仍未觀察到MUC1 mRNA轉錄上調.結論 人類呼吸道Ad5感染呼吸道上皮細胞初期,可誘導上調抑炎分子MUC1錶達,提示MUC1全部或部分參與到Ad5感染的自限性過程.但Ad7感染不能誘導上調MUC1錶達,其感染的自限性來源于其他機製.
목적 위탐토인류호흡도선병독감염자한성적분자궤제,대인류상견호흡도선병독5형(Ad5)화7형(Ad7)감염대호흡도상피세포억염분자——점단백1(MUC1)표체적영향진행초보연구,병탐토량자차이성.방법 분별용Ad7화Ad5감염호흡도상피세포A549구건감염모형.qRT-PCR화Western blot분별검측감염후MUC1 mRNA전록수평급단백표체수평변화.결과 Ad5감염A549세포후MUC1 mRNA전록수평급MUC1단백표체수평균가견상조,차정일정적시간효응;이Ad7감염후미관찰도차현상,연장Ad7감염시간후,잉미관찰도MUC1 mRNA전록상조.결론 인류호흡도Ad5감염호흡도상피세포초기,가유도상조억염분자MUC1표체,제시MUC1전부혹부분삼여도Ad5감염적자한성과정.단Ad7감염불능유도상조MUC1표체,기감염적자한성래원우기타궤제.
Objective To explore the molecular mechanism for the self-limitation of adenoviral infections in human airway,the different impacts of adenovirus serotype 5 ( Ad5 ) and serotype 7 ( Ad7 ) infections on mucin 1 ( MUC1 ) expression in airway epithelial cells were preliminarily investigated.Methods The Ad5 and the Ad7 infection models were established in A549 cell line.qRT-PCR was performed to determine the transcription of MUC1 mRNA,and the expression of MUC1 in A549 cells infected by Ad5 or Ad7 was by detected Western blot.Results An up-regulation of the MUC1 mRNA level were observed after Ad5 infection for 6 h(P＜0.05 ),and the protein expression level of MUC1 increased in a time-dependent manner in 48 hours of Ad5 infection,while similar response of MUC1 mRNA was absent in Ad7 infection (6 h),even after prolonged (20 h) treatment ( P ＞ 0.05 ).Conclusion This study reveals an up-regulation of MUC1 expression as one of the early immune response to Ad5 infection,which implies that MUC1 may function fully or partially as an anti-inflammatory factor in the self-limitation effect of Ad5 infection.However,type7 adenoviral infection,may introduce a mechanism otherwise,but through MUC1.
