CAJ | 학술논문

从水稻基因组文库中筛选得到一个水稻GST基因,命名为OsGSTL1.半定量RT-PCR分析表明OsGSTL1基因的表达不受绿磺隆、乙烯利、脱落酸、水杨酸和茉莉酸甲酯的诱导,因此该基因可能与植物抗逆性无关.为了研究OsGSTL1启动子在植物体内的表达特性,将OsGSTL1起始位点5'端上游不同长度的调控序列与报告基因GUS融合,并在洋葱表皮瞬间表达和拟南芥中稳定表达.研究表明:在洋葱表皮细胞中,160bp及更长的上游调控序列均能启动GUS基因的表达;而在转基因拟南芥中,含有2155 bp的上游序列的PGZ2.1::GUS具有时空表达的特性,在转基因的早期幼苗中GUS基因在子叶中特异性表达,但在根中没有表达;而在幼苗生长的后期,根、茎、叶中都有少量的表达.但包含1 224 bp的上游序列的PGZ1.2::GUS却表现为组成型表达的特性.由此推测,OsGSTL1启动子启动的基因表达可能与幼苗的营养代谢相关;而OsGSTL1启动子的时空表达相关元件可能位于OsGSTL1翻译起始位点5'端上游-2155 bp至-1224 bp范围内.
종수도기인조문고중사선득도일개수도GST기인,명명위OsGSTL1.반정량RT-PCR분석표명OsGSTL1기인적표체불수록광륭、을희리、탈락산、수양산화말리산갑지적유도,인차해기인가능여식물항역성무관.위료연구OsGSTL1계동자재식물체내적표체특성,장OsGSTL1기시위점5'단상유불동장도적조공서렬여보고기인GUS융합,병재양총표피순간표체화의남개중은정표체.연구표명:재양총표피세포중,160bp급경장적상유조공서렬균능계동GUS기인적표체;이재전기인의남개중,함유2155 bp적상유서렬적PGZ2.1::GUS구유시공표체적특성,재전기인적조기유묘중GUS기인재자협중특이성표체,단재근중몰유표체;이재유묘생장적후기,근、경、협중도유소량적표체.단포함1 224 bp적상유서렬적PGZ1.2::GUS각표현위조성형표체적특성.유차추측,OsGSTL1계동자계동적기인표체가능여유묘적영양대사상관;이OsGSTL1계동자적시공표체상관원건가능위우OsGSTL1번역기시위점5'단상유-2155 bp지-1224 bp범위내.
OsGSTL1 gene was isolated from the rice genomic library. Semi-quantitative RT-PCR analysis demonstrated that the expression of the OsGSTL1 in rice was not induced by chlorsulfuron, ethylene, abscisic acid, salicylic acid, and methyl jasmonate.In order to investigate the cis-elements of OsGSTL1 promoter, the promoter regions with different lengths were fused to the β-glucuronidase (GUS) reporter gene. All constructs were transformed into onion epidermal cells or A. thaliana plants to detect the expression patterns. In onion epidermal cells, the 160 bp fragment and longer ones were functional for directing GUS expression. In transgenic A. thaliana, the 2 155 bp upstream region of OsGSTL1 gene directed the GUS expression only in cotyledon after germination, but not in the root of young seedlings. In the later seedling, the 2 155 bp upstream region of OsGSTL1 gene directed GUS expression in roots, stems, and leaves. However, the GUS gene directed by a 1 224 bp upstream fragment is expressed in all the checked tissues. These results suggest that the spatiotemporal expression response elements of OsGSTL1 existed in the 5'-upstream region between -2 155 and -1 224 bp.