CAJ | 학술논문

对甘肃天水小陇山产蓝萼香茶菜进行二萜化学成分研究.采用硅胶柱色谱法分离纯化,通过薄层色谱及波谱法进行结构鉴定,SRB法分别对分离化合物进行了体外细胞毒活性测试.共鉴定出5个二萜化合物,它们分别为:1α,6,11β,15β-四乙酰基-6,7-断裂-7,20-内酯-对映-贝壳杉-16-烯(1α,6,11β,15β-tetraacetoy-6,7-seco-7,20-olide-ent-kaur-16-en,Ⅰ),6β,11α-二羟基-6,7-断裂-6,20-环氧-1α,7-内酯-对映-贝壳杉-16-烯-15-酮(6β,11α-dihydroxy-6,7-seco-6,20-epoxy-1α,7-olide-ent-kaur-16-en-15-one,Ⅱ),6β,7β,14β-三羟基-1α-乙酰氧基-7α,20-环氧-对映-贝壳杉-16烯-15-酮(6β,7β,14β-trihydroxy-1α-acetoxy-7α,20-epoxy-ent-kaur-16-en-15-one,Ⅲ),6β,11α,15α-三羟基-6,7-断裂-6,20-环氧-1α,7-内酯-对映-贝壳杉-16-烯(6β,11α,15α-trihydroxy-6,7-seco-6,20-epoxy-1α,7-olide-ent-kaur-16-en,Ⅳ),1α,6β,7β,14β-四羟基-7α,20-环氧-对映-贝壳杉-16-烯-15-酮(1α,6β,7β,14β-tetrahydroxy-7α-20-epoxy-ent-kaur-16-en-15-one,Ⅴ).化合物Ⅲ、Ⅳ、Ⅴ均为首次从该植物中分离得到,并且以上5个二萜化合物对人肝癌细胞株Bel-7402和人卵巢癌细胞株HO-8910均显示了良好的细胞毒活性.
대감숙천수소롱산산람악향다채진행이첩화학성분연구.채용규효주색보법분리순화,통과박층색보급파보법진행결구감정,SRB법분별대분리화합물진행료체외세포독활성측시.공감정출5개이첩화합물,타문분별위:1α,6,11β,15β-사을선기-6,7-단렬-7,20-내지-대영-패각삼-16-희(1α,6,11β,15β-tetraacetoy-6,7-seco-7,20-olide-ent-kaur-16-en,Ⅰ),6β,11α-이간기-6,7-단렬-6,20-배양-1α,7-내지-대영-패각삼-16-희-15-동(6β,11α-dihydroxy-6,7-seco-6,20-epoxy-1α,7-olide-ent-kaur-16-en-15-one,Ⅱ),6β,7β,14β-삼간기-1α-을선양기-7α,20-배양-대영-패각삼-16희-15-동(6β,7β,14β-trihydroxy-1α-acetoxy-7α,20-epoxy-ent-kaur-16-en-15-one,Ⅲ),6β,11α,15α-삼간기-6,7-단렬-6,20-배양-1α,7-내지-대영-패각삼-16-희(6β,11α,15α-trihydroxy-6,7-seco-6,20-epoxy-1α,7-olide-ent-kaur-16-en,Ⅳ),1α,6β,7β,14β-사간기-7α,20-배양-대영-패각삼-16-희-15-동(1α,6β,7β,14β-tetrahydroxy-7α-20-epoxy-ent-kaur-16-en-15-one,Ⅴ).화합물Ⅲ、Ⅳ、Ⅴ균위수차종해식물중분리득도,병차이상5개이첩화합물대인간암세포주Bel-7402화인란소암세포주HO-8910균현시료량호적세포독활성.