CAJ | 학술논문

为探讨茶叶糖蛋白对小鼠骨髓来源树突状细胞(BMDCs)表面分子表达的影响,采用细胞因子诱导法,以贴壁法获得贴壁单核细胞,添加重组粒细胞-巨噬细胞集落刺激因子(rmGM-CSF)和重组白细胞介素4(rmIL-4)进行体外诱导培养,倒置显微镜动态观察细胞形态的变化;采用MTT法,观察不同浓度的茶叶糖蛋白(TGP)对小鼠骨髓来源树突状细胞的细胞毒性;采用流式细胞术检测DCs的表面标志CD11c、CD86和MHC II类分子表达的变化.结果表明,经TGP作用24h后,树突状细胞形态更加典型,更加成熟;茶叶糖蛋白在(0.8～200 μg/mL)的浓度范围内,可认为TGP对树突状细胞无细胞毒性.与阴性对照相比,茶叶糖蛋白显著促进树突状细胞表面CD11c、CD86、MHC II的表达,在浓度(0.1～25 μg/mL)范围内呈现剂量依赖性,初步表明茶叶糖蛋白可以促进树突状细胞的成熟.
위탐토다협당단백대소서골수래원수돌상세포(BMDCs)표면분자표체적영향,채용세포인자유도법,이첩벽법획득첩벽단핵세포,첨가중조립세포-거서세포집락자격인자(rmGM-CSF)화중조백세포개소4(rmIL-4)진행체외유도배양,도치현미경동태관찰세포형태적변화;채용MTT법,관찰불동농도적다협당단백(TGP)대소서골수래원수돌상세포적세포독성;채용류식세포술검측DCs적표면표지CD11c、CD86화MHC II류분자표체적변화.결과표명,경TGP작용24h후,수돌상세포형태경가전형,경가성숙;다협당단백재(0.8～200 μg/mL)적농도범위내,가인위TGP대수돌상세포무세포독성.여음성대조상비,다협당단백현저촉진수돌상세포표면CD11c、CD86、MHC II적표체,재농도(0.1～25 μg/mL)범위내정현제량의뢰성,초보표명다협당단백가이촉진수돌상세포적성숙.
The effects of tea-glycoprotein (TGP) on the surface molecules expression level of murine bone marrow derived dendritic cells were observed in ouv study.Dendritic cells (DCs) generated from Balb/c murine bone marrow cells were induced by rmGM-CSF and rmIL-4.TGP was added to cells on day 6 of culture for 24 h.Morphology development of DCs was observed by invented microscope.The cytotoxicity of TGP were tested by using MTT method.The cells were analyzed by flow cytometry (FCM) to determine the proportion of CD11c cells and the change of CD86 and MHC class II molecule.The results showed that TGP treated DCs displayed a mole matured morphology,with long protrusions,while untreated DCs displayed shorter protrusions than stimulated DCs.When the concentration was below 200 μg/mL,TGP showed no cytotoxixity.TGP treated immature DCs showed an enhanced cell-surface expression of CD86 and MHC II on CD11c gated DCs,and at the concentration (0.1～25 μg/mL) was in a dose-dependent up-regulation.TGP could induce maturation of murine dendritic cells.