CAJ | 학술논문

应用聚丙烯酰胺凝胶电泳技术,研究了长毛明对虾(Fenneropenaeus penicillatus)9个野生群体杂合子缺乏及过量情况;测定了8个酶系统,共检测到15个酶位点和32个等位基因.有7个多态位点,它们是Me-1、Me-2、Mdh-2、Aat-1、Sod-2、Est-1、Amy-1位点,在这些位点有2～5个等位基因.结果表明,在长毛明对虾9个群体的全部多态位点中,共有6个位点符合哈温平衡(p＞0.05),30个位点偏离哈温平衡(p<0.05);除宁德群体外,其他8个群体中共有13个多态位点表现为杂合子缺失,结合每个群体中各个多态位点的期望杂合度与观察杂合度,认为导致杂合子缺乏的主要原因可能是种群内交、自然选择、Wahland效应、哑等位基因等.另外,在本研究中共有23个位点表现为杂合子过剩(F＜0),且在长毛明对虾9个群体中均有出现,从而认为长毛明对虾的种质资源较好.
응용취병희선알응효전영기술,연구료장모명대하(Fenneropenaeus penicillatus)9개야생군체잡합자결핍급과량정황;측정료8개매계통,공검측도15개매위점화32개등위기인.유7개다태위점,타문시Me-1、Me-2、Mdh-2、Aat-1、Sod-2、Est-1、Amy-1위점,재저사위점유2～5개등위기인.결과표명,재장모명대하9개군체적전부다태위점중,공유6개위점부합합온평형(p＞0.05),30개위점편리합온평형(p<0.05);제저덕군체외,기타8개군체중공유13개다태위점표현위잡합자결실,결합매개군체중각개다태위점적기망잡합도여관찰잡합도,인위도치잡합자결핍적주요원인가능시충군내교、자연선택、Wahland효응、아등위기인등.령외,재본연구중공유23개위점표현위잡합자과잉(F＜0),차재장모명대하9개군체중균유출현,종이인위장모명대하적충질자원교호.
Heterozygosity was investigated using the assay of vertical slab polyacrylamide gel electrophoresis in 9 populations of Fenneropenaeus penicillatus.Allozyme is co-dominant and can be analyzed under standard Hardy-Weinberg model.Allozyme electrophoresis is considered to be a critical useful technique in population genetics.Eight enzymes presumably encoded by 15 allozyme loci and 32 alleles were scored in F.penicillatus.Seven loci with 2～5 alleles are polymorphic.They are Me-1,Me-2,Mdh-2,Aat-1,Sod-2,Est-1,Amy-1.Results also showed that 6 loci of the total polymorphic loci in F.penicillatus were found to be in agreement with those expected from the Hardy-Weinberg equilibrium(p>0.05),while the other were found to depart clearly from those expected from the Hardy-Weinberg equilibrium(p<0.05).Except for Ningde Population,heterozygote deficiency existed in 13 polymorphic loci from the other 8 populations of F.penicillatus.Taking the observed heterozygosity (H_o) and the expected heterozygosity (H_e) of the population into consideration,the main reasons that lead to the heterozygote deficiency may be inbreeding,presence of null allele,Wahland effect and natural selection.Besides,heterozygote excess exists in 23 loci(F＜0) from the 9 populations of F.penicillatus,which indicated that the germplasm resource of F.penicillatus is in good conditions.