北京中医药大学学报
北京中醫藥大學學報
북경중의약대학학보
JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
2010年
1期
41-46,后插3
,共7页
赵薇%温海霞%郑慧丽%孙世晓%倪江
趙薇%溫海霞%鄭慧麗%孫世曉%倪江
조미%온해하%정혜려%손세효%예강
左归饮%P450芳香化酶%CYP19%围绝经期%卵巢%大鼠
左歸飲%P450芳香化酶%CYP19%圍絕經期%卵巢%大鼠
좌귀음%P450방향화매%CYP19%위절경기%란소%대서
Zuogui Decoction%P450 aromatase%CYP19%perimenopausal period%ovary%rats
目的 研究左归饮对围绝经期大鼠卵巢P450芳香化酶(P450 arom)及CYP19 mRNA表达的影响及机制.方法 采用围绝经期大鼠动物模型,分别给予低、中、高剂量左归饮灌胃处理8周.用放射免疫法(RIA)检测血清中雌二醇(E_2)含量;用RT-PCR和免疫组化法检测卵巢CYP19 mRNA及P450 arom的表达.未成年大鼠注射孕马血清后收集培养颗粒细胞,用RT-PCR方法检测给予不同处理后CYP19 mRNA的表达水平.结果 围绝经期大鼠血清E_2水平及卵巢P450 arom及其基因CYP19的表达明显低于青年组(P<0.01),给予中、高剂量左归饮处理后,血清E_2水平及P450 arom、CYP19表达显著升高(P<0.01).培养颗粒细胞给予不同处理因素,结果 显示,与对照组相比,染料木黄酮(Genistein)、福司考林(Forskolin)、左归饮提取物可促进CYP19 mRNA表达(P<0.05);与单纯加入左归饮提取物相比,同时加入左归饮提取物和佛波酯或Genistein可进一步促进CYP19 mRNA的表达(P<0.05).结论 中、高剂量左归饮可通过上调卵巢CYP19及其产物P450 arom的表达,使围绝经期大鼠血清E2含量升高.左归饮对CYP19 mRNA表达的促进作用可能是通过激活PKC途径或加强Genistein的作用实现的.
目的 研究左歸飲對圍絕經期大鼠卵巢P450芳香化酶(P450 arom)及CYP19 mRNA錶達的影響及機製.方法 採用圍絕經期大鼠動物模型,分彆給予低、中、高劑量左歸飲灌胃處理8週.用放射免疫法(RIA)檢測血清中雌二醇(E_2)含量;用RT-PCR和免疫組化法檢測卵巢CYP19 mRNA及P450 arom的錶達.未成年大鼠註射孕馬血清後收集培養顆粒細胞,用RT-PCR方法檢測給予不同處理後CYP19 mRNA的錶達水平.結果 圍絕經期大鼠血清E_2水平及卵巢P450 arom及其基因CYP19的錶達明顯低于青年組(P<0.01),給予中、高劑量左歸飲處理後,血清E_2水平及P450 arom、CYP19錶達顯著升高(P<0.01).培養顆粒細胞給予不同處理因素,結果 顯示,與對照組相比,染料木黃酮(Genistein)、福司攷林(Forskolin)、左歸飲提取物可促進CYP19 mRNA錶達(P<0.05);與單純加入左歸飲提取物相比,同時加入左歸飲提取物和彿波酯或Genistein可進一步促進CYP19 mRNA的錶達(P<0.05).結論 中、高劑量左歸飲可通過上調卵巢CYP19及其產物P450 arom的錶達,使圍絕經期大鼠血清E2含量升高.左歸飲對CYP19 mRNA錶達的促進作用可能是通過激活PKC途徑或加彊Genistein的作用實現的.
목적 연구좌귀음대위절경기대서란소P450방향화매(P450 arom)급CYP19 mRNA표체적영향급궤제.방법 채용위절경기대서동물모형,분별급여저、중、고제량좌귀음관위처리8주.용방사면역법(RIA)검측혈청중자이순(E_2)함량;용RT-PCR화면역조화법검측란소CYP19 mRNA급P450 arom적표체.미성년대서주사잉마혈청후수집배양과립세포,용RT-PCR방법검측급여불동처리후CYP19 mRNA적표체수평.결과 위절경기대서혈청E_2수평급란소P450 arom급기기인CYP19적표체명현저우청년조(P<0.01),급여중、고제량좌귀음처리후,혈청E_2수평급P450 arom、CYP19표체현저승고(P<0.01).배양과립세포급여불동처리인소,결과 현시,여대조조상비,염료목황동(Genistein)、복사고림(Forskolin)、좌귀음제취물가촉진CYP19 mRNA표체(P<0.05);여단순가입좌귀음제취물상비,동시가입좌귀음제취물화불파지혹Genistein가진일보촉진CYP19 mRNA적표체(P<0.05).결론 중、고제량좌귀음가통과상조란소CYP19급기산물P450 arom적표체,사위절경기대서혈청E2함량승고.좌귀음대CYP19 mRNA표체적촉진작용가능시통과격활PKC도경혹가강Genistein적작용실현적.
Objective To study the effect and regulation of Zuogui Decoction on the expressions of ovarian P450 aromatase (P450arom) and CYP19-mRNA in rats during perimenopausal period.Methods The rat model of perimenopausal period was respectively given Zuogui Decoction in high, middle and low dose orally for 8 weeks. The serum content of estradiol (E_2) was detected by using radioimmunoassay (RIA), and the expressions of ovarian CYP19-mRNA and P450arom were detected by applying RT-PCR and immunohistochemistry. The granulocytes were collected and cultured from immature rats after injection of pregnant mare serum. The expression of CYP19-mRNA with different treatments was detected by using RT-PCR. Results The serum level of E_2 and the expressions of P450arom and CYP19 were significantly lower in the perimenopausal period group than those in the young group (P<0.01), while those increased significantly (P<0.01) after the treatment with mid-dose and high-dose Zuogui Decoction. The outcomes of different treatments in cultured granulocytes showed that genistein, forskolin and extract of Zuogui Decoction improved the expression of CYP19-mRNA (P<0.05) compared with the control group. The extract of Zuogui Decoction combined with phorbol ester (PMA) or genistein improved further the expression of CYP19-mRNA (P<0.05) compared with extract of Zuogui Decoction.Conclusion The mid-dose and high-dose Zuogui Decoction can increase the serum content of E_2 through up-regulating the expressions of CYP19 and P450arom, and it also can improve the expression of CYP19-mRNA through activating PKC pathway or promoting the action of genistein in rats during perimenopausal period.
