CAJ | 학술논문

根据GenBank已发表的猪圆环病毒2 型(PCV2) 全基因组序列,设计2 对特异性引物,对广东省不同地区规模化猪场采集的疑似断奶仔猪多系统消耗综合征(PMWS) 组织病料进行了PCV2 全基因组克隆和序列分析.结果表明,PCV2 核苷酸序列较稳定,不同地区6个PCV2 毒株全基因组序列均由1 767 bp 组成,彼此间核苷酸序列相似性达95.3%～99.8%,亲缘关系密切;与GenBank 已发表的国内不同地区PCV2 参考毒株的相似性介于70.1%～99.1%;与欧洲各地区毒株的相似性介于67.7%～98.8%;其中与美国的毒株(AY094619)差异性最大,介于69.4%～70.8%之间.
근거GenBank이발표적저원배병독2 형(PCV2) 전기인조서렬,설계2 대특이성인물,대광동성불동지구규모화저장채집적의사단내자저다계통소모종합정(PMWS) 조직병료진행료PCV2 전기인조극륭화서렬분석.결과표명,PCV2 핵감산서렬교은정,불동지구6개PCV2 독주전기인조서렬균유1 767 bp 조성,피차간핵감산서렬상사성체95.3%～99.8%,친연관계밀절;여GenBank 이발표적국내불동지구PCV2 삼고독주적상사성개우70.1%～99.1%;여구주각지구독주적상사성개우67.7%～98.8%;기중여미국적독주(AY094619)차이성최대,개우69.4%～70.8%지간.
According to the genomic sequence of PCV2 published in GenBank~(TM),two pairs of primers were designed to amplify the complete genomes of PCV2 from the tissue of pigs suspected with PMWS sampled in different districts of Guangdong Province.The results showed that the complete genome of every strain consisted of 1 767 nucleotides.Nucleotide sequence homology between the six PCV2 isolates were found to be 95.3%-99.8% respectively.The homologies were70.1%-99.1% compared with the sequences of PCV2 from different regions in China published in GenBank~(TM),67.7%-98.8% as compared with the sequences of PCV2 in different regions of Europe.The homologies were 69.4%-70.8% compared with the sequence of the USA strain (AY094619),with which the variability was the biggest.