CAJ | 학술논문

通过L_9(3)~4正交设计对氯磺酸-吡啶法制备枸杞多糖硫酸酯(SLBP)的修饰条件进行优选,最佳条件为V(氯磺酸):V(吡啶)=1:4,反应温度80 ℃,反应时间2 h.枸杞多糖硫酸酯中硫的质量分数可达17.23%,硫酸基取代度可达1.935.以紫外和红外光谱对产物加以表征,并用MTT法测定了对Hela细胞体外生长的抑制作用.在25～200 mg/L内,枸杞多糖(LBP)对人宫颈癌(Hela)细胞生长几乎没有抑制作用,而枸杞多糖硫酸酯对Hela细胞的抑制率可达31.71%.用本方法所制备的枸杞多糖硫酸酯具有较高的含硫量和硫酸基取代度,对Hela细胞生长具有明显的抑制作用,其抑制率与硫酸基取代度和用药剂量呈正相关.
통과L_9(3)~4정교설계대록광산-필정법제비구기다당류산지(SLBP)적수식조건진행우선,최가조건위V(록광산):V(필정)=1:4,반응온도80 ℃,반응시간2 h.구기다당류산지중류적질량분수가체17.23%,류산기취대도가체1.935.이자외화홍외광보대산물가이표정,병용MTT법측정료대Hela세포체외생장적억제작용.재25～200 mg/L내,구기다당(LBP)대인궁경암(Hela)세포생장궤호몰유억제작용,이구기다당류산지대Hela세포적억제솔가체31.71%.용본방법소제비적구기다당류산지구유교고적함류량화류산기취대도,대Hela세포생장구유명현적억제작용,기억제솔여류산기취대도화용약제량정정상관.
The modification conditions were optimized for the preparation of sulfated lycium barbarum polysaccharide (SLBP) by using chlorsulfonic acid-pyridine method by L_9(3)~4 orthogonal design. The optimized conditions were 1∶4 of chlorosulfonic acid-topyridine, reaction temperature of 80 ℃ and reaction time of 2 h. The content of sulfur in SLBP and the degree of substitution (DS) were achieved to be 17.23% and 1.935, respectively. The nature of SLBP was characterized by UV spectra and infrared spectra analysis, and the inhibiting effect of SLBP to growth of Hela cell in vitro was tested with MTT method. In the range of 25～200 mg/L, LBP had little inhibited activity towards the Hela cells, while SLBP inhibited the growth of Hela cells remarkably with 31.71% inhibitory ratio. The SLBP prepared by the proposed sulfated method has higher sulfur content and degree of substitution, and obviously inhibiting effect to growth of Hela cells. The inhibitory ratio is positive correlation with the substitution degree and the tested dosage.