CAJ | 학술논문

目的:通过显微形态观察和功能检测分析超抗原(SEA)对Jurkat T淋巴细胞的影响.方法:应用CCK-8法,原子力显微镜(AFM)体外检测Jurkat T淋巴细胞增殖活性、及其细胞膜超微结构的改变.结果:与对照组比较,不同浓度的SEA随着作用时间的增加,细胞膜超微结构与Ra值明显发生改变.Jurkat T淋巴细胞经过质量浓度为0.1-100 mg/L SEA,作用48 h期间,Jurkat T细胞的表面超微结构(平均粗糙度,Ra)和增殖活性(A值)变化非常明显.其中,质量浓度10 mg/L SEA作用24 h,Jurkat T淋巴细胞表面结构变化最明显,而细胞增殖活性在48 h达最强.结论:SEA作为超抗原作用于Jurkat T淋巴细胞后,Jurkat T淋巴细胞的活化表现出表面形态结构改变先于代谢水平的改变,较后者更灵敏.
목적:통과현미형태관찰화공능검측분석초항원(SEA)대Jurkat T림파세포적영향.방법:응용CCK-8법,원자력현미경(AFM)체외검측Jurkat T림파세포증식활성、급기세포막초미결구적개변.결과:여대조조비교,불동농도적SEA수착작용시간적증가,세포막초미결구여Ra치명현발생개변.Jurkat T림파세포경과질량농도위0.1-100 mg/L SEA,작용48 h기간,Jurkat T세포적표면초미결구(평균조조도,Ra)화증식활성(A치)변화비상명현.기중,질량농도10 mg/L SEA작용24 h,Jurkat T림파세포표면결구변화최명현,이세포증식활성재48 h체최강.결론:SEA작위초항원작용우Jurkat T림파세포후,Jurkat T림파세포적활화표현출표면형태결구개변선우대사수평적개변,교후자경령민.
Aim:To study the effect of superantigens (SEA) on the T cell (Jurkat cell)by observing morphology and functions. Methods: Cell counting kit-8 (CCK-8), atomic force microscope(AFM) was used to check up the proliferation and membrane ultrastructure of T cell. Results: Compared to the un-treated cell, the cellular membrane ultrastructure and Ra value was significantly changed. The T cell treated with SEA(0.1～100 mg/L) for 48 hours, membrane ultrastructure Ra and proliferative activity was changed obviously. The T cell treated with 10 mg/L SEA for 24 hour, the changes of membrane ultra-structure were more remarkable, and at 48 hour the proliferation of T cell was more active. Conclusion: SEA as a superantigens, which treated T cell, and the activation of the T cell showed the morphology changes earlier than the metabolic changes.