CAJ | 학술논문

目的建立选择性山羊外周神经给乳化异氟醚模型,探索外周神经在异氟醚制动中的作用.方法 18只健康成年山羊随机平分为股动脉给乳化异氟醚组(动脉组),股动脉给脂肪乳组(动脉对照组,脂肪乳为乳化异氟醚溶剂)和耳静脉给乳化异氟醚组(静脉组).采用自身上下法,分别钳夹给药侧后肢悬蹄(动脉组,动脉对照组),后肢悬蹄(静脉组)作为伤害性刺激,测定异氟醚最低血液有效分压(minimum partial pressure, MPP).比较3组山羊不同MPP(根据取血部位不同,MPP分为颈静脉MPP、给药侧股静脉MPP和对照侧股静脉MPP).结果在动脉对照组,血液中没有发现异氟醚,山羊对钳夹刺激具有正常的逃避反应.在动脉组和静脉组,对照侧股静脉MPP与颈静脉MPP比较差异均没有统计学意义.动脉组山羊给药侧股静脉MPP约为颈静脉MPP的7倍[(38.45±17.01) mmHg vs. (5.82±2.32) mmHg,1 mmHg=0.1333 kPa, P＜0.05],约为静脉组山羊颈静脉MPP的4倍[(9.41±1.61 ) mmHg, P＜0.05].颈静脉MPP动脉组山羊约为静脉组山羊的0.6倍(P＜0.05).结论成功建立选择性山羊外周神经给乳化异氟醚模型,并提示高分压异氟醚(4倍临床麻醉深度)有外周神经阻滞作用.
목적 건립선택성산양외주신경급유화이불미모형,탐색외주신경재이불미제동중적작용.방법 18지건강성년산양수궤평분위고동맥급유화이불미조(동맥조),고동맥급지방유조(동맥대조조,지방유위유화이불미용제)화이정맥급유화이불미조(정맥조).채용자신상하법,분별겸협급약측후지현제(동맥조,동맥대조조),후지현제(정맥조)작위상해성자격,측정이불미최저혈액유효분압(minimum partial pressure, MPP).비교3조산양불동MPP(근거취혈부위불동,MPP분위경정맥MPP、급약측고정맥MPP화대조측고정맥MPP).결과 재동맥대조조,혈액중몰유발현이불미,산양대겸협자격구유정상적도피반응.재동맥조화정맥조,대조측고정맥MPP여경정맥MPP비교차이균몰유통계학의의.동맥조산양급약측고정맥MPP약위경정맥MPP적7배[(38.45±17.01) mmHg vs. (5.82±2.32) mmHg,1 mmHg=0.1333 kPa, P＜0.05],약위정맥조산양경정맥MPP적4배[(9.41±1.61 ) mmHg, P＜0.05].경정맥MPP동맥조산양약위정맥조산양적0.6배(P＜0.05).결론 성공건립선택성산양외주신경급유화이불미모형,병제시고분압이불미(4배림상마취심도)유외주신경조체작용.
Objective To develop a new model for preferential delivery of isoflurane to peripheral nerves in goats, and to identify preliminarily volatile anesthetic action sites.Methods Eighteen goats were randomly and equally divided into arterial group, control group and venous group.In the arterial group, emulsified isoflurane was infused into the femoral artery of the goats to deliver isoflurane to the peripheral nerves.In the control group, 30% Intralipid~((R) which used as a solvent of emulsified isoflurane was infused via the femoral artery of the goats with the same infusing speed as that of the arterial group.In the venous group, emulsified isoflurane was infused via an ear peripheral vein.Minimum partial pressure (MPP), the partial pressure (P_(iso)) of isoflurane in blood producing immobility in 50% of the goats exposed to noxious stimuli, was determined with an up-and-down method and a noxious stimulus by clamping the dew-claw of the hindlimbs of the goats in the arterial group and the control group, or the dew-claw of the hindlimb of the goats in the venous group.Results No isoflurane was found in the jugular and femoral veins of the goats in the control group, and normal nociceptive reflexeswere maintained.The MPP of the femoral vein of the goats from the control group did not differ from the MPP of the jugular vein of the goats from the arterial and venous groups.The MPP of femoral vein p was 7 times of that of jugular vein [(38.45±17.01) mmHg vs.(5.82±2.32) mmHg, 1 mmHg=0.1333 kPa, P<0.05] in the goats from the arterial group, and 4 times of that of jugular vein in the goats from the venous group [(9.41±1.61) mmHg, P<0.05].The MPP of jugular vein in the goats from the arterial group was about half of that of the goats in the venous group.Conclusion A new model of preferential delivery of isoflurane to the peripheral nerves in goats has been developed.Only P_(iso) higher than that used in clinical anesthetic range has a significant anesthetic effect on peripheral nerves.